Elnaggar Mahmoud M, Abdellrazeq Gaber S, Mack Victoria, Fry Lindsay M, Davis William C, Park Kun Taek
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Egypt.
Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA.
Vet Immunol Immunopathol. 2016 Oct 1;178:57-63. doi: 10.1016/j.vetimm.2016.06.010. Epub 2016 Jun 28.
The sequencing of the bovine genome and development of mass spectrometry, in conjunction with flow cytometry (FC), have afforded an opportunity to complete the characterization of the specificity of monoclonal antibodies (mAbs), only partially characterized during previous international workshops focused on antibody development for livestock (1991, Leukocyte Antigens in Cattle, Sheep, and Goats; 1993, Leukocyte Antigens of Cattle and Sheep; 1996, Third Workshop on Ruminant Leukocyte Antigens). The objective of this study was to complete the characterization of twelve mAbs incompletely characterized during the workshops that reacted with molecules predominantly expressed on bovine monocytes and use them to provide further information on the phenotypic complexity of monocyte subsets in ruminants. Analysis revealed that the mAbs could be grouped into three clusters that recognize three different molecules: CD11c, CD14, and CD163. Following characterization, comparison of the patterns of expression of CD14 and CD163 with expression of CD16, CD172a, and CD209 revealed the mononuclear cell population is comprised of multiple subsets with differential expression of these molecules. Further analysis revealed the epitopes recognized by mAbs to CD14 and CD163 are conserved on orthologues in sheep and goats. In contrast to CD14 that is also expressed on sheep and goat granulocytes, CD163 is a definitive marker for their monocytes.
牛基因组测序以及质谱技术的发展，结合流式细胞术（FC），为全面表征单克隆抗体（mAb）的特异性提供了契机。在以往专注于家畜抗体开发的国际研讨会上（1991年，牛、绵羊和山羊白细胞抗原；1993年，牛和绵羊白细胞抗原；1996年，反刍动物白细胞抗原第三次研讨会），这些单克隆抗体的特异性仅得到部分表征。本研究的目的是完成在研讨会上未完全表征的12种单克隆抗体的表征，这些抗体与主要在牛单核细胞上表达的分子发生反应，并利用它们提供有关反刍动物单核细胞亚群表型复杂性的更多信息。分析表明，这些单克隆抗体可分为三个簇，识别三种不同的分子：CD11c、CD14和CD163。表征之后，将CD14和CD163的表达模式与CD16、CD172a和CD209的表达进行比较，结果显示单核细胞群体由多个亚群组成，这些分子在亚群中的表达存在差异。进一步分析表明，针对CD14和CD163的单克隆抗体所识别的表位在绵羊和山羊的直系同源物上是保守的。与在绵羊和山羊粒细胞上也表达的CD14不同，CD163是它们单核细胞的一个明确标志物。
