Oskam J P, Dings A, van Haeringen H
Tijdschr Diergeneeskd. 1989 Jun 15;114(12):668-75.
Deaths during transport and the incidence of a poor quality of the meat in pigs were the most important reasons for studying the stress-syndrome (MHS) during the past twenty years. MHS is controlled by an autosomal Hal locus with two alleles N and n. Only pigs of genotype nn are sensitive to halothane and susceptible to stress. As a standard test for use in practice, the halothane test was developed in the Netherlands in 1974 and only this test is used to detect MHS. Heterozygotes can not be detected using this test. Since 1986, additional blood typing was carried out for the systems Phi, Po2 en Pgd in litters including at least one halothane-positive reactor. Blood typing was carried out in 533 pigs of four different lines of breeding company Cofok in Oosterhout and was found to be very successful (using the linkage groups) in identifying the heterozygotes. In 15.5 per cent of the offspring, it could not be stated whether the genotype was HalNN or HalNn. This was due to the fact that in these Nn x Nn matings the two haplotypes of one or both parents were identical. In this population, the haplotype PhiB-Po2S-PgdB was strongly (72-89 per cent) linked to the Haln-allele.
在过去二十年中，猪在运输过程中的死亡以及肉质不佳的发生率是研究应激综合征（MHS）的最重要原因。MHS由一个常染色体的Hal位点控制，该位点有两个等位基因N和n。只有基因型为nn的猪对氟烷敏感且易受应激影响。作为实际应用中的标准检测方法，氟烷检测于1974年在荷兰开发，并且仅使用该检测来检测MHS。使用此检测无法检测出杂合子。自1986年以来，对至少包含一头氟烷阳性反应猪的猪窝进行了Phi、Po2和Pgd系统的额外血型鉴定。在奥斯特豪特的育种公司Cofok的四个不同品系的533头猪中进行了血型鉴定，结果发现（利用连锁群）在鉴定杂合子方面非常成功。在15.5%的后代中，无法确定其基因型是HalNN还是HalNn。这是因为在这些Nn×Nn交配中，一个或两个亲本的两个单倍型是相同的。在这个群体中，单倍型PhiB-Po2S-PgdB与Haln等位基因紧密连锁（72-89%）。
