根瘤菌32H1中铵同化的调控
Control of ammonium assimilation in Rhizobium 32H1.
作者信息
Ludwig R A
出版信息
J Bacteriol. 1978 Jul;135(1):114-23. doi: 10.1128/jb.135.1.114-123.1978.
Abstract
The symbiotic, nitrogen-fixing bacterium Rhizobium sp. 32H1 is a specialized ammonium producer during symbiosis. However, during free-living growth, Rhizobium 32H1 assimilates ammonium very poorly. Two pathways of ammonium assimilation exist in enteric bacteria. One is mediated by glutamate dehydrogenase, and the other is mediated by glutamine synthetase-glutamate synthase. The former pathway is altogether inoperative in Rhizobium 32H1; the latter pathway operates at a slow rate and is under strict negative control by ammonium itself. Rhizobium 32H1 glutamine synthetase activity is modulated by both repression-derepression and reversible adenylylation. For a biochemical process lacking an alternative pathway, such a regulatory pattern exacerbates the very process. This suggests that Rhizobium 32H1 restricts its own ammonium assimilation to maximize the contribution of fixed nitrogen to the host plant during symbiosis.
摘要
共生固氮细菌根瘤菌属32H1在共生过程中是一种特殊的铵生产者。然而，在自由生活生长期间，根瘤菌32H1对铵的同化能力很差。肠道细菌中存在两条铵同化途径。一条由谷氨酸脱氢酶介导，另一条由谷氨酰胺合成酶-谷氨酸合酶介导。前一条途径在根瘤菌32H1中完全不起作用；后一条途径运行缓慢，并受到铵本身的严格负调控。根瘤菌32H1谷氨酰胺合成酶活性受阻遏-去阻遏和可逆腺苷酸化的调节。对于一个缺乏替代途径的生化过程来说，这样的调节模式会加剧这一过程。这表明根瘤菌32H1限制自身的铵同化，以在共生期间最大化固定氮对宿主植物的贡献。
相似文献
1
Control of ammonium assimilation in Rhizobium 32H1.根瘤菌32H1中铵同化的调控
J Bacteriol. 1978 Jul;135(1):114-23. doi: 10.1128/jb.135.1.114-123.1978.
2
Ammonium assimilation in Rhizobium meliloti.苜蓿根瘤菌中的铵同化作用。
J Bacteriol. 1980 Sep;143(3):1234-40. doi: 10.1128/jb.143.3.1234-1240.1980.
3
The pathways of ammonium assimilation in Rhizobium meliloti.苜蓿根瘤菌中铵同化的途径。
Arch Microbiol. 1981 Jul;129(5):391-4. doi: 10.1007/BF00406469.
4
Physiological roles of glutamine synthetases I and II in ammonium assimilation in Rhizobium sp. 32H1.谷氨酰胺合成酶I和II在根瘤菌32H1铵同化中的生理作用
J Bacteriol. 1980 Mar;141(3):1209-16. doi: 10.1128/jb.141.3.1209-1216.1980.
5
Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids.豆科根瘤菌类菌体中氨同化的酶
Can J Microbiol. 1975 Jul;21(7):1009-12. doi: 10.1139/m75-149.
6
Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway.菜豆根瘤菌中通过谷氨酰胺合成酶-谷氨酸合酶途径进行的铵同化作用。
J Bacteriol. 1988 Feb;170(2):980-4. doi: 10.1128/jb.170.2.980-984.1988.
7
Rhizobium sp. strain ORS571 ammonium assimilation and nitrogen fixation.根瘤菌属菌株ORS571的铵同化作用和固氮作用。
J Bacteriol. 1984 Jun;158(3):1144-51. doi: 10.1128/jb.158.3.1144-1151.1984.
8
The enzymes of the ammonia assimilation in Pseudomonas aeruginosa.铜绿假单胞菌中氨同化的酶类
Arch Microbiol. 1980 Feb;124(2-3):197-203. doi: 10.1007/BF00427727.
9
Activity of nitrogenase and glutamine synthetase in relation to availability of oxygen in continuous cultures of a strain of cowpea Rhizobium sp. supplied with excess ammonium.豇豆根瘤菌菌株连续培养物中固氮酶和谷氨酰胺合成酶的活性与氧气供应及过量铵的关系
Biochim Biophys Acta. 1978 Feb 1;538(3):406-16. doi: 10.1016/0304-4165(78)90402-6.
10
Ammonia assimilation by rhizobium cultures and bacteroids.根瘤菌培养物和类菌体对氨的同化作用。
J Gen Microbiol. 1975 Jan;86(1):39-48. doi: 10.1099/00221287-86-1-39.
引用本文的文献
1
The 3' untranslated region of the two cytosolic glutamine synthetase (GS(1)) genes in alfalfa (Medicago sativa) regulates transcript stability in response to glutamine.苜蓿（Medicago sativa）细胞质谷氨酰胺合成酶（GS(1)）的两个基因的 3'非翻译区通过响应谷氨酰胺调控转录稳定性。
Planta. 2010 Oct;232(5):1151-62. doi: 10.1007/s00425-010-1247-1. Epub 2010 Aug 13.
2
K regulates bacteroid-associated functions of Bradyrhizobium.K 调节慢生根瘤菌菌瘤相关功能。
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4650-4. doi: 10.1073/pnas.84.13.4650.
3
Regulation of Rhizobium nitrogen fixation by the unadenylylated glutamine synthetase I system.根瘤菌非腺苷酸化谷氨酰胺合成酶 I 系统对固氮的调控。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5817-21. doi: 10.1073/pnas.77.10.5817.
4
Accumulation of Amino Acids in Rhizobium sp. Strain WR1001 in Response to Sodium Chloride Salinity.根瘤菌 WR1001 菌株响应氯化钠盐度时氨基酸的积累。
Appl Environ Microbiol. 1982 Jul;44(1):135-40. doi: 10.1128/aem.44.1.135-140.1982.
5
Key role of bacterial NH(4)(+) metabolism in Rhizobium-plant symbiosis.细菌铵（NH₄⁺）代谢在根瘤菌与植物共生关系中的关键作用。
Microbiol Mol Biol Rev. 2002 Jun;66(2):203-22. doi: 10.1128/MMBR.66.2.203-222.2002.
6
Methylammonium uptake by Rhizobium sp. strain 32H1.根瘤菌属32H1菌株对甲铵的摄取
J Bacteriol. 1983 Mar;153(3):1196-201. doi: 10.1128/jb.153.3.1196-1201.1983.
7
In vitro expression of nitrogenase activity in Parasponia-Rhizobium strain ANU 289.固氮酶活性在帕拉豆根瘤菌菌株ANU 289中的体外表达。
Arch Microbiol. 1983 Jan;134(1):12-6. doi: 10.1007/BF00429399.
8
Cyclic nucleotides in procaryotes.原核生物中的环核苷酸。
Microbiol Rev. 1981 Dec;45(4):620-42. doi: 10.1128/mr.45.4.620-642.1981.
9
Rhizobium sp. strain ORS571 ammonium assimilation and nitrogen fixation.根瘤菌属菌株ORS571的铵同化作用和固氮作用。
J Bacteriol. 1984 Jun;158(3):1144-51. doi: 10.1128/jb.158.3.1144-1151.1984.
10
Cloning of the glutamine synthetase I gene from Rhizobium meliloti.来自苜蓿根瘤菌的谷氨酰胺合成酶I基因的克隆。
J Bacteriol. 1983 Oct;156(1):168-76. doi: 10.1128/jb.156.1.168-176.1983.
本文引用的文献
1
Symposium on multiple forms of enzymes and control mechanisms. I. Multiple forms of enzymes.酶的多种形式与调控机制研讨会。I. 酶的多种形式。
Bacteriol Rev. 1963 Jun;27(2):155-69. doi: 10.1128/br.27.2.155-169.1963.
2
Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules.大豆根瘤匀浆中类菌体部分的固氮作用。
Biochim Biophys Acta. 1967 Aug 29;141(3):507-15. doi: 10.1016/0304-4165(67)90179-1.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
'Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP); an enzyme involved in the synthesis of glutamate by some bacteria.谷氨酰胺（酰胺）：2-氧化戊二酸氨基转移酶氧化还原酶（NADP）；一种参与某些细菌合成谷氨酸的酶。
J Gen Microbiol. 1970 Dec;64(2):187-94. doi: 10.1099/00221287-64-2-187.
5
The regulation of glutamine synthesis in microorganisms.微生物中谷氨酰胺合成的调控。
Annu Rev Microbiol. 1970;24:501-24. doi: 10.1146/annurev.mi.24.100170.002441.
6
Effects of specific divalent cations on some physical and chemical properties of glutamine synthetase from Escherichia coli. Taut and relaxed enzyme forms.特定二价阳离子对大肠杆菌谷氨酰胺合成酶某些物理和化学性质的影响。紧密型和松弛型酶形式。
Biochemistry. 1968 Jun;7(6):2153-67. doi: 10.1021/bi00846a018.
7
Regulation of glutamine synthetase. 8. ATP: glutamine synthetase adenylyltransferase, an enzyme that catalyzes alterations in the regulatory properties of glutamine synthetase.谷氨酰胺合成酶的调节。8. ATP：谷氨酰胺合成酶腺苷酰转移酶，一种催化谷氨酰胺合成酶调节特性改变的酶。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1703-10. doi: 10.1073/pnas.58.4.1703.
8
Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein.来自大肠杆菌的谷氨酸合酶。一种铁硫黄素蛋白。
J Biol Chem. 1972 Nov 25;247(22):7407-19.
9
Glutamate synthase: properties of the reduced nicotinamide adenine dinucleotide-dependent enzyme from Saccharomyces cerevisiae.谷氨酸合酶：来自酿酒酵母的依赖还原型烟酰胺腺嘌呤二核苷酸的酶的特性
J Bacteriol. 1974 Apr;118(1):89-95. doi: 10.1128/jb.118.1.89-95.1974.
10
5'-adenylyl-O-tyrosine. The novel phosphodiester residue of adenylylated glutamine synthetase from Escherichia coli.5'-腺苷酰-O-酪氨酸。来自大肠杆菌的腺苷酰化谷氨酰胺合成酶的新型磷酸二酯残基。
J Biol Chem. 1968 Jul 10;243(13):3769-71.
Zotero 插件