豆科根瘤菌类菌体中氨同化的酶

Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids.

作者信息

Kurz W G, Rokosh D A, LaRue T A

出版信息

Can J Microbiol. 1975 Jul;21(7):1009-12. doi: 10.1139/m75-149.

Abstract

The activities of the following enzymes were studied in connection with dinitrogen fixation in pea bacteroids: glutamine synthetase(L-glutamate: ammonia ligase (ADP-forming)(EC 6.3.1.2)(GS); glutamate dehydrogenase (NADP+)(L-glutamate: NADP+ oxidoreductase (deaminating)(EC 1.4.1.4)(GDH); glutamate synthase (L-glutamine: 2-exeglutarate aminotransferase (NADPH-oxidizing))(EC 2.6.1.53)(GOGAT). GS activity was high throughout the growth of the plant and GOGAT activity was always low. It is unlikely that GDH or the GS-GOGAT pathway can account for the incorporation of ammonia from dinitrogen fixation in the pea bacteroid,

摘要

结合豌豆类菌体中的固氮作用，对以下几种酶的活性进行了研究：谷氨酰胺合成酶（L-谷氨酸：氨连接酶（生成ADP）（EC 6.3.1.2）（GS））；谷氨酸脱氢酶（NADP⁺）（L-谷氨酸：NADP⁺氧化还原酶（脱氨基）（EC 1.4.1.4）（GDH））；谷氨酸合酶（L-谷氨酰胺：2-酮戊二酸氨基转移酶（氧化NADPH））（EC 2.6.1.53）（GOGAT）。在植株整个生长过程中，GS活性都很高，而GOGAT活性始终很低。GDH或GS-GOGAT途径不太可能解释豌豆类菌体中来自固氮作用的氨的掺入情况。