Martin Stephen B, Schauer Elizabeth S, Blum David H, Kremer Paul A, Bahnfleth William P, Freihaut James D
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Respiratory Health Division, Field Studies Branch, 1095 Willowdale Road, Morgantown, WV 26505, USA; Pennsylvania State University, College of Engineering, Department of Architectural Engineering, Indoor Environment Center, 104 Engineering Unit A, University Park, PA 16802, USA.
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Respiratory Health Division, Field Studies Branch, 1095 Willowdale Road, Morgantown, WV 26505, USA.
J Photochem Photobiol B. 2016 Sep;162:674-680. doi: 10.1016/j.jphotobiol.2016.07.028. Epub 2016 Jul 25.
We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) real-time UV dose (fluence) determination; and 2) simple actinometric determination of a reactor factor that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This reactor factor replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k=0.1471cm(2)/mJ (with 95% confidence bounds of 0.1426 to 0.1516).
我们开发、表征并测试了一种新型双准直水性紫外线反应器，以提高水性k值测定的准确性和一致性。这种新系统很独特，因为它能将来自单个灯的紫外线能量沿两个相反方向准直。与传统的单准直系统相比，该设计具有两个明显优势：1）实时紫外线剂量（通量）测定；2）简单的光化测定反应器因子，该因子将测量的辐照度水平与微生物悬浮液实际经历的辐照度水平相关联。这个反应器因子取代了单准直反应器所需的四个典型校正因子中的三个。使用这种双准直反应器，枯草芽孢杆菌孢子呈现出符合经典多击模型的失活情况，k = 0.1471平方厘米/毫焦（95%置信区间为0.1426至0.1516）。
