从美国国防部血清库检测血清微小RNA：与可替宁、细胞因子和多环芳烃水平的相关性
Detection of Serum microRNAs From Department of Defense Serum Repository: Correlation With Cotinine, Cytokine, and Polycyclic Aromatic Hydrocarbon Levels.
作者信息
Woeller Collynn F, Thatcher Thomas H, Van Twisk Daniel, Pollock Stephen J, Croasdell Amanda, Kim Nina, Hopke Philip K, Xia Xiaoyan, Thakar Juilee, Mallon Col Timothy M, Utell Mark J, Phipps Richard P
机构信息
Department of Environmental Medicine (Dr Woeller, Mr Pollock, Ms Croasdell, Ms Kim, Dr Utell, Dr Phipps), Department of Medicine (Drs Thatcher, Utell, and Phipps), Microbiology and Immunology, University of Rochester Medical Center (Mr Van Twisk, Drs Thakar and Phipps); Center for Air Resources Engineering and Science, Clarkson University, Potsdam, New York (Drs Hopke and Xia); and Professor, Department of Preventive Medicine and Biostatistics, Uniformed Services University, Bethesda, MD 20814 (Dr Mallon).
出版信息
J Occup Environ Med. 2016 Aug;58(8 Suppl 1):S62-71. doi: 10.1097/JOM.0000000000000742.
OBJECTIVE
The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs).
METHODS
MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy.
RESULTS
We detected miRNAs, cytokines, IgE, and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine suggesting tobacco exposure. Significant associations between serum cotinine, cytokine, IgE, PAHs, and miRNA were discovered.
CONCLUSION
We successfully quantified over 200 potential biomarkers of occupational exposure from DoDSR samples. The stored serum samples were not affected by hemolysis and represent a powerful tool for biomarker discovery and analysis in retrospective studies.
目的
本研究旨在调查美国国防部血清库（DoDSR）的血清样本质量是否足以检测微小RNA（miRNA）、细胞因子、免疫球蛋白E（IgE）和多环芳烃（PAH）。
方法
通过聚合酶链反应（PCR）阵列分离并定量miRNA。使用多重免疫测定法测量与炎症相关的细胞因子和趋化因子。通过酶联免疫吸附测定（ELISA）检测可替宁和IgE，通过液相色谱/质谱法检测PAH。
结果
我们以高灵敏度检测到了miRNA、细胞因子、IgE和PAH。30个样本中有11个可替宁检测呈阳性，表明有烟草接触史。发现血清可替宁、细胞因子、IgE、PAH和miRNA之间存在显著关联。
结论
我们成功地从DoDSR样本中定量了200多种潜在的职业暴露生物标志物。储存的血清样本未受溶血影响，是回顾性研究中生物标志物发现和分析的有力工具。
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