Kouris Eleftherios, Kalogiannis Stavros, Perdih Franc, Turel Iztok, Psomas George
Department of General and Inorganic Chemistry, Faculty of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Department of Nutrition and Dietetics, Faculty of Agriculture, Food Technology and Nutrition, Alexander Technological Educational Institution, Sindos, Thessaloniki, Greece.
J Inorg Biochem. 2016 Oct;163:18-27. doi: 10.1016/j.jinorgbio.2016.07.022. Epub 2016 Jul 28.
The cobalt(II) complexes with the quinolone sparfloxacin (Hsf) in the absence or presence of the nitrogen-donor heterocyclic ligands 2,2'-bipyridine (bipy), 1,10-phenanthroline (phen) or 2,2'-bipyridylamine (bipyam) were prepared and characterized physicochemically and spectroscopically. The crystal structures of complexes [Co(sf)(bipy)]∙3MeOH∙2HO and [Co(sf)(phen)]∙4MeOH were determined by X-ray crystallography. The antimicrobial activity of the complexes was tested against four different microorganisms (Escherichia coli, Xanthomonas campestris, Staphylococcus aureus and Bacillus subtilis) and was found similar or higher than that of free Hsf. The binding of the complexes to calf-thymus DNA was monitored by UV-vis spectroscopy and DNA-viscosity measurements and indirectly by competitive studies with ethidium bromide; intercalation is suggested as the most possible interaction mode. Fluorescence emission spectroscopy was used to evaluate the interaction of the complexes with human or bovine serum albumin and the corresponding binding constants were determined.
制备了钴（II）与喹诺酮类药物司帕沙星（Hsf）在不存在或存在含氮杂环配体2,2'-联吡啶（bipy）、1,10-菲咯啉（phen）或2,2'-联吡啶胺（bipyam）情况下形成的配合物，并通过物理化学和光谱方法对其进行了表征。通过X射线晶体学确定了配合物[Co(sf)(bipy)]∙3MeOH∙2HO和[Co(sf)(phen)]∙4MeOH的晶体结构。测试了这些配合物对四种不同微生物（大肠杆菌、野油菜黄单胞菌、金黄色葡萄球菌和枯草芽孢杆菌）的抗菌活性，发现其与游离Hsf的抗菌活性相似或更高。通过紫外可见光谱和DNA粘度测量以及通过与溴化乙锭的竞争研究间接监测了配合物与小牛胸腺DNA的结合；插层被认为是最可能的相互作用模式。使用荧光发射光谱法评估了配合物与人或牛血清白蛋白的相互作用，并确定了相应的结合常数。
