Dawane Jayshree Shriram, Pandit Vijaya Anil, Deshpande Swapnil Suryakant, Mandpe Amruta Sumedh
Assistant Professor, Department of Pharmacology, BVDU Medical College , Pune, India .
Professor, Department of Pharmacology, BVDU Medical College , Pune, India .
J Clin Diagn Res. 2016 Jun;10(6):FF01-4. doi: 10.7860/JCDR/2016/17940.7912. Epub 2016 Jun 1.
Diabetes is a metabolic disease of vital health importance because of the complications associated with it. Clinical trials and animal studies have demonstrated the anti-hyperglycaemic effect of Nishamalaki. Present study was planned to evaluate the protective potential of Nishamalaki on diabetic complication in rats.
To study the Nephro-protective effect and to assess the protective potential on retinal changes of Nishamalaki in diabetic wistar rats.
Diabetes induced with 60 mg/kg of Streptozotocin and 110 mg/kg Nicotinamide IP. Nishamalaki, a combination of Curcuma longa and Emblica officinalis administered orally with honey. Rats divided into six groups, control and diabetic rats with blood glucose above 250 mg/dl were divided into 5 groups. After 8 weeks test animals were treated with Nishamalaki, Enalapril and control with saline for 30 days. Biochemical parameters measured like Serum BSL, BUN and Creatinine and rats were observed for development of cataract. Rats sacrificed and kidney samples were taken to examine histopathological changes.
Blood Urea Nitrogen and Creatinine values were significantly (p<0.01) reduced in Nishamalaki group than control group. Nishamalaki showed the protective effect on kidney pathology as seen on histopathology by near normal glomerular and tubular structures. Control group showed shrunken glomerulus and tubular vacuolations. In Nishamalaki group immature sub capsular cataract with mild lenticular opacity were seen compared to the mature cataract with significant lenticular opacity and corneal vascularisation in control group.
Nishamalaki showed protective effect on development of Nephrotoxicity and it has also delayed the progression of cataract in rats.
糖尿病是一种具有重要健康意义的代谢性疾病，因其会引发多种并发症。临床试验和动物研究已证实尼沙玛拉基（Nishamalaki）具有抗高血糖作用。本研究旨在评估尼沙玛拉基对大鼠糖尿病并发症的保护潜力。
研究尼沙玛拉基对糖尿病wistar大鼠的肾脏保护作用，并评估其对视网膜变化的保护潜力。
通过腹腔注射60mg/kg链脲佐菌素和110mg/kg烟酰胺诱导糖尿病。尼沙玛拉基由姜黄和余甘子组合而成，与蜂蜜一起口服给药。将大鼠分为六组，血糖高于250mg/dl的对照组和糖尿病大鼠分为5组。8周后，对试验动物用尼沙玛拉基、依那普利治疗，并用生理盐水作为对照，持续30天。测量血清血糖、尿素氮和肌酐等生化参数，并观察大鼠白内障的发展情况。处死大鼠后，取肾脏样本检查组织病理学变化。
尼沙玛拉基组的血尿素氮和肌酐值较对照组显著降低（p<0.01）。从组织病理学上看，尼沙玛拉基对肾脏病理具有保护作用，肾小球和肾小管结构接近正常。对照组显示肾小球萎缩和肾小管空泡化。与对照组出现的成熟白内障伴有明显晶状体混浊和角膜血管化相比，尼沙玛拉基组可见未成熟的囊下白内障伴轻度晶状体混浊。
尼沙玛拉基对肾毒性的发展具有保护作用，并且还延缓了大鼠白内障的进展。
