Pünchera Jöri, Barnes Laurent, Kaya Gürkan
Department of Dermatology, University Hospital of Geneva, Geneva, Switzerland.
Dermatopathology (Basel). 2016 Jun 1;3(2):44-54. doi: 10.1159/000446427. eCollection 2016 Apr-Jun.
Sebaceous glands contribute significantly to the barrier functions of the skin. However, little is known about their homeostasis and tumorigenesis. Recently, increased expression of stem cell marker Lrig1 has been reported in sebaceous carcinoma-like tumors of K14ΔNLef1 transgenic mice. In this study, we analyzed the Lrig1 expression in human sebaceous tumors.
Twenty-eight formalin-fixed paraffin-embedded sebaceous tumor specimens (7 sebaceous hyperplasias, 7 sebaceous adenomas, 10 sebaceomas and 4 sebaceous carcinomas) were stained with anti-Lrig1, anti-CD44v3 and anti-Ki67 antibody.
Four (100%) sebaceous carcinomas, 8 (80%) sebaceomas, 3 (43%) sebaceous adenomas and no sebaceous hyperplasia showed Lrig1 overexpression.
Lrig1 is a known tumor suppressor gene and is usually considered to be an indicator of poorly aggressive tumors. In human sebaceous tumors, the stronger Lrig1 staining in sebaceous carcinoma compared to other sebaceous tumors might be a feature of an advanced stage in tumorigenesis and a bad prognosis. In our study, 100% of sebaceous carcinomas revealed Lrig1 overexpression. We propose that Lrig1 may be used as a possible new marker of poorly differentiated sebaceous carcinoma.
皮脂腺对皮肤的屏障功能有重要作用。然而，关于其稳态和肿瘤发生知之甚少。最近，有报道称在K14ΔNLef1转基因小鼠的皮脂腺癌样肿瘤中干细胞标志物Lrig1的表达增加。在本研究中，我们分析了Lrig1在人类皮脂腺肿瘤中的表达情况。
用抗Lrig1、抗CD44v3和抗Ki67抗体对28例福尔马林固定石蜡包埋的皮脂腺肿瘤标本（7例皮脂腺增生、7例皮脂腺腺瘤、10例皮脂腺瘤和4例皮脂腺癌）进行染色。
4例（100%）皮脂腺癌、8例（80%）皮脂腺瘤、3例（43%）皮脂腺腺瘤显示Lrig1过表达，皮脂腺增生均未显示Lrig1过表达。
Lrig1是一种已知的肿瘤抑制基因，通常被认为是侵袭性较弱肿瘤的一个指标。在人类皮脂腺肿瘤中，与其他皮脂腺肿瘤相比，皮脂腺癌中Lrig1染色更强可能是肿瘤发生晚期的一个特征及预后不良的表现。在我们的研究中，100%的皮脂腺癌显示Lrig1过表达。我们认为Lrig1可能用作低分化皮脂腺癌一种可能的新标志物。
