Department of Surgery, Section of Pathology, University of Cagliari, Cagliari, 09100 Sardinia, Italy.
ELPEN Experimental Center, Athens, Greece.
Biomed Res Int. 2016;2016:7261960. doi: 10.1155/2016/7261960. Epub 2016 Jul 18.
Aim. To evaluate the effects of erythropoietin administration on the adrenal glands in a swine model of ventricular fibrillation and resuscitation. Methods. Ventricular fibrillation was induced via pacing wire forwarded into the right ventricle in 20 female Landrace/Large White pigs, allocated into 2 groups: experimental group treated with bolus dose of erythropoietin (EPO) and control group which received normal saline. Cardiopulmonary resuscitation (CPR) was performed immediately after drug administration as per the 2010 European Resuscitation Council (ERC) guidelines for Advanced Life Support (ALS) until return of spontaneous circulation (ROSC) or death. Animals who achieved ROSC were monitored, mechanically ventilated, extubated, observed, and euthanized. At necroscopy, adrenal glands samples were formalin-fixed, paraffin-embedded, and routinely processed. Sections were stained with hematoxylin-eosin. Results. Oedema and apoptosis were the most frequent histological changes and were detected in all animals in the adrenal cortex and in the medulla. Mild and focal endothelial lesions were also detected. A marked interindividual variability in the degree of the intensity of apoptosis and oedema at cortical and medullary level was observed within groups. Comparing the two groups, higher levels of pathological changes were detected in the control group. No significant difference between the two groups was observed regarding the endothelial changes. Conclusions. In animals exposed to ventricular fibrillation, EPO treatment has protective effects on the adrenal gland.
目的。评估促红细胞生成素给药对心室颤动和复苏猪模型肾上腺的影响。方法。通过将起搏线推进右心室在 20 头雌性长白/大白猪中诱导心室颤动，将其分为 2 组：实验组给予促红细胞生成素（EPO）推注剂量，对照组给予生理盐水。给药后立即按照 2010 年欧洲复苏委员会（ERC）高级生命支持（ALS）指南进行心肺复苏（CPR），直至自主循环恢复（ROSC）或死亡。达到 ROSC 的动物进行监测、机械通气、拔管、观察和安乐死。在尸检时，肾上腺样本用福尔马林固定、石蜡包埋并常规处理。切片用苏木精-伊红染色。结果。水肿和细胞凋亡是最常见的组织学变化，在肾上腺皮质和髓质的所有动物中均有发现。还检测到轻度和局灶性的内皮损伤。在组内观察到皮质和髓质水平细胞凋亡和水肿程度的个体间变异性很大。比较两组，对照组的病理变化程度更高。两组间内皮变化无显著差异。结论。在暴露于心室颤动的动物中，EPO 治疗对肾上腺具有保护作用。
