Lu Ying, Ni Sujie, He Li-Na, Gao Yong-Jing, Jiang Bao-Chun
Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, Jiangsu 226001, China; Pain Research Laboratory, Institute of Nautical Medicine, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
Department of Medical Oncology, The affiliated Hospital of Nantong University, Jiangsu 226001, China.
Neurosci Lett. 2016 Sep 19;631:1-6. doi: 10.1016/j.neulet.2016.08.009. Epub 2016 Aug 6.
ANXA10 (annexin A10) is a member of the annexin family, and its biological effects are mediated primarily through the calcium-dependent phospholipid-binding and calcium ion binding. We examined the gene expressions of the L5 spinal cord after spinal nerve ligation (SNL)-induced neuropathic pain in mice by gene chip. The results showed that Anxa10 mRNA was the most upregulated gene in annexin family with 73.7-fold increase. Although previous studies have reported that several annexins are involved in nociceptive pain, the role of Anxa10 in pain remains undefined. We aimed to evaluate the role of ANXA10 in mediating injury-induced heat hyperalgesia and mechanical allodynia. We found that SNL induced persistent upregulation of Anxa10 mRNA and protein in the spinal cord of mice. Moreover, ANXA10 was colocalized with the neuronal marker MAP2 and astrocytic marker glial fibrillary acidic protein (GFAP), but not with microglial marker CD11b. Finally, pretreatment with Anxa10 siRNA partially prevented SNL-induced mechanical allodynia and heat hyperalgesia. Posttreatment with Anxa10 siRNA attenuated SNL-induced neuropathic pain. These findings suggest that ANXA10 might be a novel target in the treatment of neuropathic pain.
膜联蛋白A10（ANXA10）是膜联蛋白家族的成员，其生物学效应主要通过钙依赖性磷脂结合和钙离子结合介导。我们通过基因芯片检测了小鼠脊髓神经结扎（SNL）诱导的神经性疼痛后L5脊髓的基因表达。结果显示，Anxa10 mRNA是膜联蛋白家族中上调最为明显的基因，增加了73.7倍。尽管先前的研究报道几种膜联蛋白参与伤害性疼痛，但Anxa10在疼痛中的作用仍不明确。我们旨在评估ANXA10在介导损伤诱导的热痛觉过敏和机械性异常性疼痛中的作用。我们发现SNL诱导小鼠脊髓中Anxa10 mRNA和蛋白持续上调。此外，ANXA10与神经元标志物微管相关蛋白2（MAP2）和星形胶质细胞标志物胶质纤维酸性蛋白（GFAP）共定位，但与小胶质细胞标志物CD11b不共定位。最后，用Anxa10小干扰RNA（siRNA）预处理可部分预防SNL诱导的机械性异常性疼痛和热痛觉过敏。用Anxa10 siRNA后处理可减轻SNL诱导的神经性疼痛。这些发现表明，ANXA10可能是治疗神经性疼痛的一个新靶点。
