用二氧化钛和炭黑处理后的人肺上皮细胞A549蛋白质组数据。
Human lung epithelial cell A549 proteome data after treatment with titanium dioxide and carbon black.
作者信息
Vuong Ngoc Q, Goegan Patrick, Mohottalage Susantha, Breznan Dalibor, Ariganello Marianne, Williams Andrew, Elisma Fred, Karthikeyan Subramanian, Vincent Renaud, Kumarathasan Premkumari
机构信息
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9; Department of Biochemistry, Faculty of Science, University of Ottawa, Ottawa, ON, Canada K1H 8M5.
Inhalation Toxicology Laboratory, Environmental Health Science and Research Bureau, Health Canada, Ottawa, ON, Canada K1A 0K9.
出版信息
Data Brief. 2016 Jun 21;8:687-91. doi: 10.1016/j.dib.2016.06.013. eCollection 2016 Sep.
Here, we have described the dataset relevant to the A549 cellular proteome changes after exposure to either titanium dioxide or carbon black particles as compared to the non-exposed controls, "Proteomic changes in human lung epithelial cells (A549) in response to carbon black and titanium dioxide exposures" (Vuong et al., 2016) [1]. Detailed methodologies on the separation of cellular proteins by 2D-GE and the subsequent mass spectrometry analyses using MALDI-TOF-TOF-MS are documented. Particle exposure-specific protein expression changes were measured via 2D-GE spot volume analysis. Protein identification was done by querying mass spectrometry data against SwissProt and RefSeq protein databases using Mascot search engine. Two-way ANOVA analysis data provided information on statistically significant A549 protein expression changes associated with particle exposures.
在此，我们描述了与A549细胞蛋白质组在暴露于二氧化钛或炭黑颗粒后相对于未暴露对照组的变化相关的数据集，“人类肺上皮细胞（A549）对炭黑和二氧化钛暴露的蛋白质组变化”（Vuong等人，2016年）[1]。记录了通过二维凝胶电泳分离细胞蛋白质以及随后使用基质辅助激光解吸电离飞行时间串联质谱进行质谱分析的详细方法。通过二维凝胶电泳斑点体积分析测量颗粒暴露特异性蛋白质表达变化。使用Mascot搜索引擎通过查询针对SwissProt和RefSeq蛋白质数据库的质谱数据来进行蛋白质鉴定。双向方差分析数据提供了与颗粒暴露相关的A549蛋白质表达变化具有统计学意义的信息。
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