小鼠肝脏的快速二维差异凝胶电泳蛋白质组学分析

Rapid 2D DIGE Proteomic Analysis of Mouse Liver.

作者信息

Kamata Shotaro, Ishii Isao

机构信息

Laboratory of Health Chemistry, Showa Pharmaceutical University, Machida, Tokyo, 194-8543, Japan.

出版信息

Methods Mol Biol. 2018;1664:153-166. doi: 10.1007/978-1-4939-7268-5_13.

DOI:10.1007/978-1-4939-7268-5_13

PMID:29019132

Abstract

Several years have passed since LC-MS(/MS) became the mainstream for proteomic analysis; however, conventional 2D DIGE (two-dimensional difference gel electrophoresis) continues to be an important technology that enables rapid and direct visualization of hundreds to thousands of proteins and their quantitative analyses. We can get global proteomic views using 2D DIGE within 3 days, and then identify proteins with differential expression levels using MALDI-TOF/MS and MASCOT search engine within a week. Here, we describe our routine 2D DIGE proteomic analysis of the liver isolated from mice in pathological conditions.

摘要

液相色谱-质谱联用（/串联质谱）成为蛋白质组学分析的主流技术已有数年；然而，传统的二维差异凝胶电泳（2D DIGE）仍然是一项重要技术，它能够快速、直接地可视化数百至数千种蛋白质及其定量分析。我们可以在3天内使用2D DIGE获得整体蛋白质组学视图，然后在一周内使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/MS）和MASCOT搜索引擎鉴定表达水平有差异的蛋白质。在此，我们描述了我们对病理条件下从小鼠分离的肝脏进行的常规二维差异凝胶电泳蛋白质组学分析。