Liu Jie, Fu Ruiqi, Liu Ranran, Zhao Guiping, Zheng Maiqing, Cui Huanxian, Li Qinghe, Song Jiao, Wang Jie, Wen Jie
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China.
PLoS One. 2016 Aug 10;11(8):e0159722. doi: 10.1371/journal.pone.0159722. eCollection 2016.
Muscle development and growth influences the efficiency of poultry meat production, and is closely related to deposition of intramuscular fat (IMF), which is crucial in meat quality. To clarify the molecular mechanisms underlying muscle development and IMF deposition in chickens, protein expression profiles were examined in the breast muscle of Beijing-You chickens at ages 1, 56, 98 and 140 days, using isobaric tags for relative and absolute quantification (iTRAQ). Two hundred and four of 494 proteins were expressed differentially. The expression profile at day 1 differed greatly from those at day 56, 98 and 140. KEGG pathway analysis of differential protein expression from pair-wise comparisons (day 1 vs. 56; 56 vs. 98; 98 vs. 140), showed that the fatty acid degradation pathway was more active during the stage from day 1 to 56 than at other periods. This was consistent with the change in IMF content, which was highest at day 1 and declined dramatically thereafter. When muscle growth was most rapid (days 56-98), pathways involved in muscle development were dominant, including hypertrophic cardiomyopathy, dilated cardiomyopathy, cardiac muscle contraction, tight junctions and focal adhesion. In contrast with hatchlings, the fatty acid degradation pathway was downregulated from day 98 to 140, which was consistent with the period for IMF deposition following rapid muscle growth. Changes in some key specific proteins, including fast skeletal muscle troponin T isoform, aldehyde dehydrogenase 1A1 and apolipoprotein A1, were verified by Western blotting, and could be potential biomarkers for IMF deposition in chickens. Protein-protein interaction networks showed that ribosome-related functional modules were clustered in all three stages. However, the functional module involved in the metabolic pathway was only clustered in the first stage (day 1 vs. 56). This study improves our understanding of the molecular mechanisms underlying muscle development and IMF deposition in chickens.
肌肉发育和生长影响禽肉生产效率，并且与肌内脂肪（IMF）沉积密切相关，而肌内脂肪对肉质至关重要。为阐明鸡肌肉发育和IMF沉积的分子机制，利用相对和绝对定量等压标签（iTRAQ）技术，检测了1、56、98和140日龄北京油鸡胸肌中的蛋白质表达谱。494种蛋白质中有204种表达存在差异。1日龄时的表达谱与56、98和140日龄时的差异很大。对两两比较（1日龄与56日龄；56日龄与98日龄；98日龄与140日龄）的差异蛋白质表达进行KEGG通路分析，结果表明，脂肪酸降解通路在1日龄至56日龄阶段比其他时期更活跃。这与IMF含量的变化一致，IMF含量在1日龄时最高，此后急剧下降。当肌肉生长最快时（56 - 98日龄），参与肌肉发育的通路占主导地位，包括肥厚型心肌病、扩张型心肌病、心肌收缩、紧密连接和粘着斑。与雏鸡相比，脂肪酸降解通路从98日龄到140日龄下调，这与快速肌肉生长后的IMF沉积时期一致。通过蛋白质免疫印迹法验证了一些关键特异性蛋白质的变化，包括快肌肌钙蛋白T同工型、醛脱氢酶1A1和载脂蛋白A1，这些蛋白质可能是鸡IMF沉积的潜在生物标志物。蛋白质 - 蛋白质相互作用网络表明，核糖体相关功能模块在所有三个阶段都有聚类。然而，参与代谢通路的功能模块仅在第一阶段（1日龄与56日龄）聚类。本研究增进了我们对鸡肌肉发育和IMF沉积分子机制的理解。
