Martins Waleska B, Rodrigues Sheyla A, Silva Hatamy K, Dantas Camila G, Júnior Waldecy DE Lucca, Filho Lauro Xavier, Cardoso Juliana C, Gomes Margarete Z
Instituto de Tecnologia e Pesquisa/ITP, Av. Murilo Dantas, 300, Farolândia, 49032-490 Aracaju, SE, Brasil.
Universidade Federal de Sergipe, Cidade Universitária Prof. José Aloísio de Campos, Av. Marechal Rondon, s/n, Jardim Rosa Elze, 49100-000 São Cristóvão, SE, Brasil.
An Acad Bras Cienc. 2016 Sep;88(3):1439-50. doi: 10.1590/0001-3765201620150574. Epub 2016 Aug 4.
The Portulaca oleracea L. (Portulacaceae) is a cosmopolitan species with a wide range of biological activities, including antioxidant and neuroprotective actions. We investigated the effects of P. oleracea extracts in a 6-hydroxydopamine rat model of Parkinson's disease, a debilitating disorder without effective treatments. Chemical profiles of aqueous and ethanolic extracts of whole plant were analyzed by thin layer chromatography and the antioxidant activity was assessed by 2,2-diphenyl-1-picrilhidrazila method. Male Wistar rats received intrastriatal 6-hydroxydopamine and were treated with vehicle or extracts (oral, 200 and 400 mg/kg) daily for two weeks. The behavioral open field test was conducted at days 1 and 15. Immunohistochemical analysis was performed 4 weeks after surgery to quantify tyrosine-hydroxylase cell counts in the substantia nigra pars compacta. Extracts presented antioxidant activity in concentrations above 300 µg/kg. The chromatographic analysis revealed the presence of Levodopa, alkaloids, flavonoids, saponins, tannins, terpenoids and polysaccharides. Both extracts improved motor recovery 15 days after lesion and protected from tyrosine-hydroxylase cell loss after 4 weeks, but these effects were more evident for the aqueous extract. Because the dopamine precursor is present, in addition to antioxidant compounds and neuroprotective effects, P. oleracea can be considered as potential strategy for treating Parkinson's disease.
马齿苋（马齿苋科）是一种分布广泛的世界性物种，具有多种生物活性，包括抗氧化和神经保护作用。我们在帕金森病的6-羟基多巴胺大鼠模型中研究了马齿苋提取物的作用，帕金森病是一种使人衰弱且尚无有效治疗方法的疾病。通过薄层色谱法分析了全株植物水提取物和乙醇提取物的化学特征，并采用2,2-二苯基-1-苦基肼法评估了抗氧化活性。雄性Wistar大鼠接受纹状体内注射6-羟基多巴胺，并每天用赋形剂或提取物（口服，200和400mg/kg）处理两周。在第1天和第15天进行行为旷场试验。术后4周进行免疫组织化学分析，以量化黑质致密部酪氨酸羟化酶细胞计数。提取物在浓度高于300μg/kg时具有抗氧化活性。色谱分析显示存在左旋多巴、生物碱、黄酮类、皂苷、单宁、萜类和多糖。两种提取物均能在损伤后15天改善运动恢复，并在4周后防止酪氨酸羟化酶细胞丢失，但水提取物的这些作用更为明显。由于除了抗氧化化合物和神经保护作用外还存在多巴胺前体，马齿苋可被视为治疗帕金森病的潜在策略。
