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纳米孔-CMOS 接口用于 DNA 测序。

Nanopore-CMOS Interfaces for DNA Sequencing.

机构信息

Department of Electrical Engineering and Computer Science, York University, 6400 Keele St, Toronto, ON M3J-1P3, Canada.

出版信息

Biosensors (Basel). 2016 Aug 6;6(3):42. doi: 10.3390/bios6030042.

DOI:10.3390/bios6030042
PMID:27509529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5039661/
Abstract

DNA sequencers based on nanopore sensors present an opportunity for a significant break from the template-based incumbents of the last forty years. Key advantages ushered by nanopore technology include a simplified chemistry and the ability to interface to CMOS technology. The latter opportunity offers substantial promise for improvement in sequencing speed, size and cost. This paper reviews existing and emerging means of interfacing nanopores to CMOS technology with an emphasis on massively-arrayed structures. It presents this in the context of incumbent DNA sequencing techniques, reviews and quantifies nanopore characteristics and models and presents CMOS circuit methods for the amplification of low-current nanopore signals in such interfaces.

摘要

基于纳米孔传感器的 DNA 测序仪为摆脱过去四十年来基于模板的主流技术提供了一个重要的机会。纳米孔技术带来的主要优势包括简化的化学方法和与 CMOS 技术接口的能力。后者为提高测序速度、尺寸和成本提供了巨大的潜力。本文综述了现有的和新兴的纳米孔与 CMOS 技术接口的方法，重点介绍了大规模阵列结构。本文在现有 DNA 测序技术的背景下进行了介绍，综述和量化了纳米孔的特性和模型，并提出了 CMOS 电路方法，用于在这种接口中放大低电流纳米孔信号。

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