Wang Yuxi, Guo Qiang, Chen Hongwei, Chen Minghua, Yang Sigang, Xie Shizhong
Opt Lett. 2016 Aug 15;41(16):3755-8. doi: 10.1364/OL.41.003755.
An imaging strategy based on optical time-encoded structured illumination microscopy (TE-SIM) opens the way toward ultrafast superresolution imaging. A proof-of-principle experiment is conducted and the introduced TE-SIM accelerates the generation rate of sinusoidal fringe patterns to an unprecedented speed (dozens of megahertz). At such a high speed, superresolution imaging that surpasses the diffraction limit by a factor of 1.4 is demonstrated. This imaging strategy with high temporal and spatial resolution has great potential in many exciting applications, such as dynamic live cell imaging or high-throughput screening.
一种基于光学时间编码结构照明显微镜（TE-SIM）的成像策略为超快超分辨率成像开辟了道路。开展了一项原理验证实验，所引入的TE-SIM将正弦条纹图案的生成速率提升至前所未有的速度（数十兆赫兹）。在如此高的速度下，实现了超越衍射极限1.4倍的超分辨率成像。这种具有高时间和空间分辨率的成像策略在许多令人兴奋的应用中具有巨大潜力，如动态活细胞成像或高通量筛选。
