Engel Ulrike
Center for Organismal Studies and Nikon Imaging Center, Bioquant, University of Heidelberg, Heidelberg, Germany.
Methods Cell Biol. 2014;123:315-33. doi: 10.1016/B978-0-12-420138-5.00017-3.
Structured illumination microscopy (SIM) with a 3-dimensional illumination pattern allows to double image resolution laterally and axially. For cell biologists, SIM may become an attractive tool for refined colocalization studies and to investigate the assembly of components at higher resolution. In this chapter, we focus on the use of a commercial available SIM setup and provide guidance on sample preparation and image acquisition. We present superresolution images of the cytoskeleton in fixed cells and discuss the potential and limitations for SIM in live imaging.
具有三维照明模式的结构照明显微镜（SIM）能够在横向和轴向上将图像分辨率提高一倍。对于细胞生物学家而言，SIM可能会成为一种有吸引力的工具，用于精细的共定位研究以及以更高分辨率研究组件的组装。在本章中，我们重点介绍商用SIM装置的使用，并提供样品制备和图像采集方面的指导。我们展示了固定细胞中细胞骨架的超分辨率图像，并讨论了SIM在活细胞成像中的潜力和局限性。
