酵母甾醇酯化作用的体外测定

Measurement in vitro of the esterification of yeast sterols.

作者信息

Parks L W, Stromberg V K

机构信息

Department of Microbiology, Oregon State University, 97331, Corvallis, Oregon.

出版信息

Lipids. 1978 Jan;13(1):29-33. doi: 10.1007/BF02533363.

DOI:10.1007/BF02533363

PMID:27519996

Abstract

A highly reproducible and rapid procedure is described for isolating free sterols and sterol esters from cell-free extracts of yeast. Using this technique, the sterol esterifying enzyme has been studied. Sterol ester hydrolase has also been demonstrated. In agreement with whole cell experiments, the enzyme activity for sterol ester synthesis has been found to increase on entrance of the culture into the stationary phase of growth.

摘要

本文描述了一种从酵母无细胞提取物中分离游离甾醇和甾醇酯的高度可重复且快速的方法。利用该技术，对甾醇酯化酶进行了研究。同时也证实了甾醇酯水解酶的存在。与全细胞实验结果一致，发现甾醇酯合成酶活性在培养物进入生长稳定期时增加。