National Heart and Lung Institute, Imperial College London, Heart Science Centre, Harefield, Middlesex, UB9 6JH, UK.
Mol Cell Biochem. 2004 Aug;263(1):81-90. doi: 10.1023/B:MCBI.0000041850.37415.b8.
The recent completion of a first draft of the human genome has allowed "in silico" genome browsing to become routine. Such computer-based research is now a useful adjunct to experiments based at the bench, and is accelerating gene discovery and the analysis and understanding of genes in their genomic contexts. This review summarises recent findings on genes encoding proteins of the troponin complex. We describe the organization of the three pairs of genes which encode isoforms of troponins I and T, and discuss how this relates to their evolution and regulation. Detailed analysis of the chromosomal context of the cardiac troponin I and slow skeletal troponin T genes reveals a region of densely packed differentially expressed genes, including new genes identified by automatic genome annotation. This information is discussed within the context of detailed analysis of the best-studied gene in this region, cardiac troponin I. In this way, we illustrate the uses to which a combination of conventional bench experiments and "in silico" analyses may be put in understanding the relationship between structure and function within the genome. (Mol Cell Biochem 263: 81-90, 2004).
人类基因组初稿的最近完成使得“计算机上”的基因组浏览成为常规。这种基于计算机的研究现在是基于实验台的实验的有用补充，并加速了基因的发现以及对基因在其基因组环境中的分析和理解。这篇综述总结了最近关于编码肌钙蛋白复合物蛋白的基因的发现。我们描述了编码肌钙蛋白 I 和 T 同工型的三对基因的组织，并讨论了它们的进化和调控如何与之相关。对心脏肌钙蛋白 I 和慢骨骼肌肌钙蛋白 T 基因的染色体环境的详细分析揭示了一个包含大量差异表达基因的区域，包括通过自动基因组注释鉴定的新基因。在对该区域中研究最深入的基因——心脏肌钙蛋白 I 的详细分析的背景下，讨论了这些信息。通过这种方式，我们说明了在理解基因组内结构与功能之间的关系时，传统的实验台实验和“计算机上”分析的组合的用途。（分子细胞生物化学 263:81-90, 2004）。
