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[分光光度法测定溶液中的蛋白质浓度]

[Spectrophotometric determination of the protein concentration in solutions].

作者信息

Fedenko V S, Vinnichenko A N, Shenkarenko I V, Syrovatko V A

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1989(4):108-12.

PMID:2752068
Abstract

The spectrophotometric methods for determination of protein concentration in solution are analysed. According to the given data it has been concluded that the method based on the measurement of difference in light absorption by protein solution at 235 and 280 nm is more accurate as compared with the method of absorption determination at 260 and 280 nm. The linear regression coefficient is used in calculation.

摘要

分析了用于测定溶液中蛋白质浓度的分光光度法。根据所给数据得出结论,与在260和280nm处测定吸光度的方法相比,基于测量蛋白质溶液在235和280nm处光吸收差异的方法更准确。计算中使用了线性回归系数。

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