Vekshin N L
Nauchnye Doki Vyss Shkoly Biol Nauki. 1988(4):107-11.
A combined method for spectrophotometric measurement of protein amount for conventionally isolated biological suspensions is suggested. The method is based on the comparison of data on the optical density of a suspension at 280-290 nm with the result of a single colorimetric determination. For the membranes of sarcoplasmic reticulum and mitochondria it has been shown that the combined spectrophotometric method gives possibility to shorten the time of measurement, has high specificity and sensitivity and compares favourably with usual colorimetric methods in relation to accuracy. A calibration chart was obtained for conventionally isolated "Wistar" rat liver mitochondria.
提出了一种用于分光光度法测量常规分离的生物悬浮液中蛋白质含量的组合方法。该方法基于将悬浮液在280 - 290nm处的光密度数据与单次比色测定结果进行比较。对于肌浆网和线粒体的膜，已表明组合分光光度法能够缩短测量时间，具有高特异性和灵敏度，并且在准确性方面与常规比色法相比具有优势。获得了常规分离的“Wistar”大鼠肝线粒体的校准图。
