Sox 蛋白与基因组结合的分子基础。

Molecular basis for the genome engagement by Sox proteins.

机构信息

Genome Regulation Laboratory, Drug Discovery Pipeline, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.

出版信息

Semin Cell Dev Biol. 2017 Mar;63:2-12. doi: 10.1016/j.semcdb.2016.08.005. Epub 2016 Aug 10.

DOI:10.1016/j.semcdb.2016.08.005

PMID:27521520

Abstract

The Sox transcription factor family consists of 20 members in the human genome. Many of them are key determinants of cellular identities and possess the capacity to reprogram cell fates by pioneering the epigenetic remodeling of the genome. This activity is intimately tied to their ability to specifically bind and bend DNA alone or with other proteins. Here we discuss our current knowledge on how Sox transcription factors such as Sox2, Sox17, Sox18 and Sox9 'read' the genome to find and regulate their target genes and highlight the roles of partner factors including Pax6, Nanog, Oct4 and Brn2. We integrate insights from structural and biochemical studies as well as high-throughput assays to probe DNA specificity in vitro as well as in cells and tissues.

摘要

Sox 转录因子家族由人类基因组中的 20 个成员组成。它们中的许多成员是细胞身份的关键决定因素，并且具有通过开拓基因组的表观遗传重塑来重新编程细胞命运的能力。这种活性与它们单独或与其他蛋白质结合并弯曲 DNA 的能力密切相关。在这里，我们讨论了我们目前对 Sox2、Sox17、Sox18 和 Sox9 等 Sox 转录因子如何“读取”基因组以发现和调节其靶基因的了解，并强调了包括 Pax6、Nanog、Oct4 和 Brn2 在内的伙伴因子的作用。我们整合了结构和生化研究以及高通量测定的见解，以探测体外以及细胞和组织中的 DNA 特异性。