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猪脑匀浆含有平滑肌肌球蛋白和细胞质肌球蛋白同工型。

Pig brain homogenates contain smooth muscle myosin and cytoplasmic myosin isoforms.

作者信息

Gröschel-Stewart U, Magel E, Paul E, Neidlinger A C

机构信息

Institut für Zoologie, Technische Hochschule, Darmstadt, Federal Republic of Germany.

出版信息

Cell Tissue Res. 1989 Jul;257(1):137-9. doi: 10.1007/BF00221643.

DOI:10.1007/BF00221643
PMID:2752402
Abstract

Three myosin isoforms, two of smooth muscle and one of cytoplasmic origin, were found in porcine brain by Western blotting analysis with antibodies specific for smooth and cytoplasmic myosins. The smooth muscle isoforms comprise at least 30% of the total myosin present. Brain tissue is therefore not a suitable source for the isolation of pure cytoplasmic contractile proteins.

摘要

通过使用针对平滑肌和细胞质肌球蛋白的特异性抗体进行蛋白质免疫印迹分析,在猪脑中发现了三种肌球蛋白亚型,其中两种起源于平滑肌,一种起源于细胞质。平滑肌亚型至少占总肌球蛋白的30%。因此,脑组织不是分离纯细胞质收缩蛋白的合适来源。

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本文引用的文献

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Immunization, isolation of immunoglobulins, estimation of antibody titre.免疫接种、免疫球蛋白的分离、抗体滴度的测定。
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