Paul E R, Christian A L, Franke R, Gröschel-Stewart U
Institut für Zoologie, Technische Hochschule Darmstadt, Germany.
Cell Tissue Res. 1994 May;276(2):381-6. doi: 10.1007/BF00306123.
Antibodies to smooth muscle and non-muscle myosin allow the development of smooth muscle and its capillary system in the embryonic chicken gizzard to be followed by immunofluorescent techniques. Although smooth muscle development proceeds in a serosal to luminal direction, angiogenetic cell clusters develop independently at the luminal side close to the epithelial layer, and the presumptive capillaries invade the developing muscle in a luminal to serosal direction. The smooth muscle and non-muscle myosin heavy chains in this avian system cannot be separated by SDS polyacrylamide gel electrophoresis and do not show isoform specificity in immunoblotting, unlike the system found in mammals. Only two myosin heavy chains with M(r) of 200 and 196 kDa were separable and considerable immunological cross-reactivity was found between the denatured myosin isoform heavy chains.
针对平滑肌和非肌肉肌球蛋白的抗体，使利用免疫荧光技术追踪胚胎期鸡砂囊平滑肌及其毛细血管系统的发育成为可能。尽管平滑肌的发育是从浆膜向管腔方向进行的，但血管生成细胞簇在靠近上皮层的管腔侧独立发育，并且推测的毛细血管以管腔向浆膜的方向侵入正在发育的肌肉。与哺乳动物系统不同，该禽类系统中的平滑肌和非肌肉肌球蛋白重链不能通过SDS聚丙烯酰胺凝胶电泳分离，并且在免疫印迹中不显示同工型特异性。只有两条分子量分别为200 kDa和196 kDa的肌球蛋白重链可以分离，并且在变性的肌球蛋白同工型重链之间发现了相当程度的免疫交叉反应。
