Department of Biomedical Engineering, National University of Singapore, Singapore.
Interdisciplinary Graduate School, Nanyang Technological University, Singapore.
Nanomedicine (Lond). 2016 Sep;11(17):2275-87. doi: 10.2217/nnm-2016-0173. Epub 2016 Aug 16.
We examined the impact of aggregation and protein corona formation of gold nanoparticles (AuNPs) on the cytotoxicity, uptake and metabolism, specifically urea and albumin synthesis, of primary rat hepatocytes.
MATERIALS & METHODS: The AuNPs were synthesized via citrate reduction and the human serum protein corona was preformed on the AuNPs. Primary hepatocytes were isolated from male Wistar rats via two-step in situ collagenase perfusion method, and were dosed with both citrate-capped (AuNP-Cit) and protein corona coated AuNPs (AuNP-Cor).
The AuNP-Cor showed higher cell uptake and reduced cell viability compared with aggregated AuNP-Cit. Urea and albumin secretions showed AuNP dose dependency. Both AuNP-Cit and AuNP-Cor exerted only an acute effect on the albumin synthesis of hepatocytes with no chronic impact.
研究金纳米粒子（AuNPs）聚集和蛋白冠形成对原代大鼠肝细胞的细胞毒性、摄取和代谢（特别是尿素和白蛋白合成）的影响。
通过柠檬酸还原法合成 AuNPs，并在 AuNPs 上预先形成人血清蛋白冠。通过两步原位胶原酶灌注法从雄性 Wistar 大鼠中分离原代肝细胞，并分别用柠檬酸封端的 AuNPs（AuNP-Cit）和蛋白冠包被的 AuNPs（AuNP-Cor）处理。
与聚集的 AuNP-Cit 相比，AuNP-Cor 表现出更高的细胞摄取和降低的细胞活力。尿素和白蛋白分泌表现出 AuNP 剂量依赖性。AuNP-Cit 和 AuNP-Cor 对肝细胞的白蛋白合成仅表现出急性作用，没有慢性影响。
