Université de Lorraine, CITHEFOR, Nancy, France; Université de Strasbourg, Faculty of Medicine, Inserm UMR 1121 Biomaterials and Bioengineering, Strasbourg, France.
Université de Strasbourg, Faculty of Medicine, Inserm UMR 1121 Biomaterials and Bioengineering, Strasbourg, France.
Int J Pharm. 2020 Apr 30;580:119244. doi: 10.1016/j.ijpharm.2020.119244. Epub 2020 Mar 19.
Gold nanoparticle (AuNP) interaction with the blood compartment as a function of their charge and the binding energy of their surface ligand was explored. Citrate, polyallylamine and cysteamine stabilized AuNP along with dihydrolipoic acid and polyethylene glycol capped AuNP were synthesized and fully characterized. Their interactions with model proteins (human albumin and human fibrinogen) were studied. Complexes formed between AuNP and protein revealed several behaviors ranging from corona formation to aggregation. Protein fluorescence quenching as a function of temperature and AuNP concentration allowed the determination of the thermodynamic parameters describing these interactions. The hemolysis induced by AuNP was also probed: an increasing or a decreasing of hemolysis ratio induced by AuNP was observed as of function of protein corona formation. Taken together, our results drew up a composite sketch of an ideal surface ligand for blood compatible AuNP. This capping agent should be strongly bound to the gold core by one or more thiol groups and it must confer a negative charge to the particles.
研究了金纳米粒子（AuNP）与血液成分的相互作用，这取决于它们的电荷和表面配体的结合能。合成并全面表征了柠檬酸盐、聚烯丙胺和半胱胺稳定的 AuNP 以及二氢硫辛酸和聚乙二醇封端的 AuNP。研究了它们与模型蛋白（人血清白蛋白和人纤维蛋白原）的相互作用。AuNP 与蛋白质形成的复合物表现出多种行为，从冠形成到聚集。作为温度和 AuNP 浓度函数的蛋白质荧光猝灭允许确定描述这些相互作用的热力学参数。还研究了 AuNP 诱导的溶血：随着蛋白质冠形成，观察到 AuNP 诱导的溶血率增加或减少。总之，我们的结果描绘了一种理想的血液相容 AuNP 表面配体的综合草图。这种封端剂应该通过一个或多个硫醇基团与金核强烈结合，并且必须赋予粒子负电荷。
