Hao Xiaoli, Hu Jiao, Wang Jiongjiong, Xu Jing, Cheng Hao, Xu Yunpeng, Li Qunhui, He Dongchang, Liu Xiaowen, Wang Xiaoquan, Gu Min, Hu Shunlin, Xu Xiulong, Liu Huimou, Chen Sujuan, Peng Daxin, Liu Xiufan
Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Animal Infectious Disease Laboratory, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China.
Vet Microbiol. 2016 Aug 30;192:95-101. doi: 10.1016/j.vetmic.2016.07.002. Epub 2016 Jul 7.
Reassortment between different influenza viruses is a crucial way to generate novel influenza viruses with unpredictable virulence and transmissibility, which may threaten the public health. As currently in China, avian influenza viruses (AIVs) of H9N2 and H5N1 subtypes are endemic in poultry in many areas, while they are prone to reassort with each other naturally. In order to evaluate the risk of the reassortment to public health, A/Goose/Jiangsu/k0403/2010 [GS/10(H5N1)] virus was used as a backbone to generate a series of reassortants, each contained a single internal gene derived from the predominant S genotype of the A/Chicken/Jiangsu/WJ57/2012 [WJ/57(H9N2)]. We next assessed the biological characteristics of these assortments, including pathogenicity, replication efficiency and polymerase activity. We found that the parental WJ/57(H9N2) and GS/10(H5N1) viruses displayed high genetic compatibility. Notably, the H5N1 reassortants containing the PA or NP gene from WJ/57(H9N2) virus significantly increased virulence and replication ability in mice, as well as markedly enhanced polymerase activity. Our results indicate that the endemicity of H9N2 and H5N1 in domestic poultry greatly increases the possibility of generating new viruses by reassortment that may pose a great threat to poultry industry and public health.
不同流感病毒之间的基因重配是产生具有不可预测毒力和传播性的新型流感病毒的关键途径，这可能会威胁公众健康。目前在中国，H9N2和H5N1亚型禽流感病毒（AIVs）在许多地区的家禽中呈地方流行性，并且它们很容易自然地相互基因重配。为了评估基因重配对公众健康的风险，以A/Goose/Jiangsu/k0403/2010 [GS/10(H5N1)]病毒为骨架构建了一系列重配病毒，每个重配病毒都包含一个来自A/Chicken/Jiangsu/WJ57/2012 [WJ/57(H9N2)]主要S基因型的单个内部基因。接下来，我们评估了这些重配病毒的生物学特性，包括致病性、复制效率和聚合酶活性。我们发现亲本WJ/57(H9N2)和GS/10(H5N1)病毒表现出高度的基因兼容性。值得注意的是，含有来自WJ/57(H9N2)病毒PA或NP基因的H5N1重配病毒在小鼠中的毒力和复制能力显著增加，并且聚合酶活性也明显增强。我们的结果表明，H9N2和H5N1在家禽中的地方流行性大大增加了通过基因重配产生新病毒的可能性，这可能对家禽业和公众健康构成巨大威胁。
