Laurentie M P, Barenton B, Charrier J, Garcia-Villar R, Marnet P G, Blanchard M, Toutain P L
INRA, Station de Pharmacologie-Toxicologie, Toulouse, France.
Endocrinology. 1989 Aug;125(2):642-51. doi: 10.1210/endo-125-2-642.
Relationships among sleep, feeding behavior, posture, and GH secretion were investigated in two groups of ruminant lambs in January (n = 6) and May (n = 3). Lambs were placed in individual cages and fed ad libitum. Behavioral features were obtained from continuous polygraphic recording. Blood was collected from undisturbed sheep every 3 min for 24 h via an indwelling catheter connected to a peristaltic pump. One month after the sampling session, ovine GH (oGH) was iv administered to evaluate oGH kinetic parameters. From GH plasma concentrations and oGH kinetic parameters, the instantaneous secretion rate of GH was reconstituted using a numerical deconvolution method. All lambs exhibited normal behavioral patterns. The clearance of oGH was similar for the two groups, and the daily production rates of GH were estimated at 14.60 +/- 7.99 micrograms/kg.24 h in January and 10.57 +/- 5.21 micrograms/kg.24 h in May. Analysis of concentration profiles indicate an episodic pattern of GH secretion into plasma. The mean number of peaks was 16.22 +/- 4.47/24 h, and the mean duration was 47.2 +/- 12.8 min for the nine sheep. When instantaneous secretion rates were taken into account, the number of identified peaks was similar, but the mean duration was reduced (32.9 +/- 9.8 min for the nine sheep). Significant relationships were not found between GH plasma concentration profiles and the state of vigilance, food behavior, or posture. Conversely, when the instantaneous secretion rates were taken into account, the highest GH production rate was detected during rest, i.e. slow wave sleep and rapid eye movement sleep, absence of food intake or rumination, and lying down. It is emphasized that the use of GH instantaneous secretion rate instead of GH concentration is of importance when evaluating the relationships between GH dynamics and short duration events. It is concluded that the influence of vigilance on GH secretion, which has already been demonstrated in humans, is likely to exist in other species.
在1月（n = 6）和5月（n = 3）对两组反刍羊羔的睡眠、进食行为、姿势和生长激素（GH）分泌之间的关系进行了研究。将羊羔置于单独的笼子中，自由采食。行为特征通过连续的多导记录获得。通过连接蠕动泵的留置导管，在24小时内每隔3分钟从安静的绵羊身上采集一次血液。采样期结束后1个月，静脉注射羊生长激素（oGH）以评估oGH动力学参数。根据GH血浆浓度和oGH动力学参数，使用数值反卷积方法重建GH的瞬时分泌率。所有羊羔均表现出正常的行为模式。两组的oGH清除率相似，1月GH的日分泌率估计为14.60±7.99微克/千克·24小时，5月为10.57±5.21微克/千克·24小时。浓度曲线分析表明GH分泌到血浆中呈间歇性模式。9只绵羊的平均峰值数量为16.22±4.47/24小时，平均持续时间为47.2±12.8分钟。当考虑瞬时分泌率时，识别出的峰值数量相似，但平均持续时间缩短（9只绵羊为32.9±9.8分钟）。未发现GH血浆浓度曲线与警觉状态、进食行为或姿势之间存在显著关系。相反，当考虑瞬时分泌率时，在休息期间，即慢波睡眠和快速眼动睡眠、无进食或反刍以及躺下时，检测到最高的GH分泌率。强调在评估GH动态与短时间事件之间的关系时，使用GH瞬时分泌率而非GH浓度非常重要。得出的结论是，警觉对GH分泌的影响在人类中已得到证实，在其他物种中可能也存在。
