Moorthy Anandi Narayana, Tan Kong Bing, Wang Shi, Narasaraju Teluguakula, Chow Vincent T
Department of Microbiology and Immunology, School of Medicine, National University of Singapore, National University Health System , Singapore.
Department of Pathology, National University Hospital, National University of Singapore, National University Health System , Singapore.
Front Immunol. 2016 Aug 2;7:289. doi: 10.3389/fimmu.2016.00289. eCollection 2016.
Obesity is an independent risk factor for severe outcome of influenza infection. Higher dietary fat consumption has been linked to greater morbidity and severe influenza in mouse models. However, the extent of generation of neutrophil extracellular traps (NETs or NETosis) in obese individuals during influenza pneumonia is hitherto unknown. This study investigated pulmonary NETs generation in BALB/c mice fed with high-fat diet (HFD) and low-fat diet (LFD), during the course of influenza pneumonia. Clinical disease progression, histopathology, lung reactive oxygen species, and myeloperoxidase activity were also compared. Consumption of HFD over 18 weeks led to significantly higher body weight, body mass index, and adiposity in BALB/c mice compared with LFD. Lethal challenge of mice (on HFD and LFD) with influenza A/PR/8/34 (H1N1) virus led to similar body weight loss and histopathologic severity. However, NETs were formed at relatively higher levels in mice fed with HFD, despite the absence of significant difference in disease progression between HFD- and LFD-fed mice.
肥胖是流感感染严重后果的独立危险因素。在小鼠模型中，较高的膳食脂肪摄入量与更高的发病率和严重流感相关。然而，肥胖个体在流感肺炎期间中性粒细胞胞外陷阱（NETs或NETosis）的生成程度迄今尚不清楚。本研究调查了在流感肺炎病程中，高脂饮食（HFD）和低脂饮食（LFD）喂养的BALB/c小鼠肺部NETs的生成情况。还比较了临床疾病进展、组织病理学、肺活性氧和髓过氧化物酶活性。与LFD相比，18周以上食用HFD导致BALB/c小鼠体重、体重指数和肥胖程度显著更高。用甲型/PR/8/34（H1N1）流感病毒对（HFD和LFD喂养的）小鼠进行致死性攻击导致相似的体重减轻和组织病理学严重程度。然而，尽管HFD喂养和LFD喂养的小鼠在疾病进展方面没有显著差异，但HFD喂养的小鼠中NETs的形成水平相对较高。
