Ishaq Muhammad, Razi Raziuddin, Khan Sabaz Ali
Department of Plant Breeding and Genetics, the University of Agriculture, Peshawar, Pakistan.
Department of Environmental Sciences, COMSATS Institute of Information Technology, 22060, Abbottabad, Pakistan.
J Sci Food Agric. 2017 Apr;97(6):1924-1930. doi: 10.1002/jsfa.7997. Epub 2016 Sep 21.
Development of new genotypes having high oil content and desirable levels of fatty acid compositions is a major objective of rapeseed breeding programmes. In the current study combining ability was determined for oil, protein, glucosinolates and various fatty acids content using 8 × 8 full diallel in rapeseed (Brassica napus).
Highly significant genotypic differences were observed for oil, protein, glucosinolates, oleic acid, linolenic acid and erucic acid content. Mean squares due to general combining ability (GCA), specific combining ability (SCA) and reciprocal combining ability (RCA) were highly significant (P ≤ 0.01) for biochemical traits. Parental line AUP-17 for high oil content and low glucosinolates, genotype AUP-2 for high protein and oleic acids, and AUP-18 for low lenolenic and erucic acid were best general combiners. Based on desirable SCA effects, F hybrids AUP-17 × AUP-20; AUP-2 × AUP-8; AUP-7 × AUP-14; AUP-2 × AUP-9; AUP-7 × AUP-14 and AUP-2 × AUP-9 were found superior involving at least one best general combiner.
F hybrids AUP-17 × AUP-20 (for oil content); AUP-2 × AUP-8 (for protein content); AUP-7 × AUP-14 (for glucosinolates); AUP-2 × AUP-9 (for oleic acid); AUP-7 × AUP-14 (for linolenic acid) and AUP-2 × AUP-9 (for erucic acid) were found superior involving at least one best general combiner. As reciprocal crosses of AUP-14 with AUP-7 and AUP-8 were superior had low × low and low × high GCA effects for glucosinolates and oleic acid, respectively therefore, these could be exploited in future rapeseed breeding programmes to develop new lines with good quality. © 2016 Society of Chemical Industry.
培育具有高含油量和理想脂肪酸组成水平的新基因型是油菜育种计划的主要目标。在本研究中，利用8×8完全双列杂交法测定了油菜（甘蓝型油菜）中油、蛋白质、硫代葡萄糖苷和各种脂肪酸含量的配合力。
观察到油、蛋白质、硫代葡萄糖苷、油酸、亚麻酸和芥酸含量存在极显著的基因型差异。生化性状的一般配合力（GCA）、特殊配合力（SCA）和反交配合力（RCA）的均方极显著（P≤0.01）。高含油量和低硫代葡萄糖苷的亲本系AUP - 17、高蛋白和油酸的基因型AUP - 2以及低亚麻酸和芥酸的AUP - 18是最佳的一般配合者。基于理想的SCA效应，F1杂种AUP - 17×AUP - 20；AUP - 2×AUP - 8；AUP - 7×AUP - 14；AUP - 2×AUP - 9；AUP - 7×AUP - 14和AUP - 2×AUP - 9被发现表现优异，且至少涉及一个最佳一般配合者。
F1杂种AUP - 17×AUP - 20（含油量）；AUP - 2×AUP - 8（蛋白质含量）；AUP - 7×AUP - 14（硫代葡萄糖苷）；AUP - 2×AUP - 9（油酸）；AUP - 7×AUP - 14（亚麻酸）和AUP - 2×AUP - 9（芥酸）被发现表现优异，且至少涉及一个最佳一般配合者。由于AUP - 14与AUP - 7和AUP - 8的反交组合表现优异，硫代葡萄糖苷和油酸的GCA效应分别为低×低和低×高，因此，这些组合可用于未来的油菜育种计划，以培育优质新品系。©2016化学工业协会。
