School of Food Science and Engineering, South China University of Technology , Guangzhou 510640, China.
School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou 510640, China.
J Agric Food Chem. 2016 Aug 31;64(34):6533-8. doi: 10.1021/acs.jafc.6b02675. Epub 2016 Aug 19.
In this study, a novel two-step enzymatic reaction was developed for the synthesis of highly pure triacylglycerols (TAGs) with a high content of n-3 polyunsaturated fatty acids (PUFAs). Glyceride mixtures were primarily synthesized by Novozym 435-catalyzed transesterification of glycerol and DHA/EPA-rich ethyl esters (EEs), followed by removal of partial glycerides, for the first time, by immobilized mono- and diacylglycerol lipase SMG1-F278N-catalyzed ethanolysis. TAG yield as high as 98.66% was achieved under the optimized conditions, and highly pure (98.75%) n-3 PUFA-enriched TAGs with 88.44% of n-3 PUFA was obtained after molecular distillation at lower temperature (140 °C). In addition, the EEs produced during ethanolysis had a FA composition similar to that of the original EEs, making them feasible for cyclic utilization. This was the first study reporting removal of partial glycerides by ethanolysis. Through ethanolysis, a higher purity product could be easily obtained at a relatively low temperature compared with the conventional high-temperature molecular distillation.
在这项研究中，开发了一种新颖的两步酶促反应，用于合成高纯度含有高含量 n-3 多不饱和脂肪酸 (PUFA) 的三酰基甘油 (TAG)。甘油和富含 DHA/EPA 的乙酯 (EE) 的 Novozym 435 催化的转酯化反应首先合成甘油酯混合物，然后首次通过固定化单酰基和二酰基甘油脂肪酶 SMG1-F278N 催化的乙醇解去除部分甘油酯。在优化条件下，TAG 的产率高达 98.66%，并且在较低温度（140°C）下通过分子蒸馏后，可获得高度纯净（98.75%）的 n-3 PUFA 富集 TAG，其 n-3 PUFA 含量为 88.44%。此外，乙醇解过程中产生的 EE 具有与原始 EE 相似的 FA 组成，使其可循环利用。这是首次报道通过乙醇解去除部分甘油酯的研究。与传统的高温分子蒸馏相比，通过乙醇解可以在相对较低的温度下更容易获得更高纯度的产物。
