Yui Kunio, Tanuma Nasoyuki, Yamada Hiroshi, Kawasaki Yohei
Department of Pediatrics, Dokkyo Medical University, 880 Kitakobayashi, Mibu 321-0293, Tochigi, Japan; Department of Drug Evaluation and Informatics, School of Pharmaceutical Science, University of Shizuoka, Shizuoka 422-8526, Japan.
Department of Pediatrics, Tokyo Metropolitan Fuchu Medical Center for the Disabled, Tokyo 183-8553, Japan.
Int J Dev Neurosci. 2017 Aug;60:70-77. doi: 10.1016/j.ijdevneu.2016.08.003. Epub 2016 Aug 21.
Individuals with autism spectrum disorders (ASD) have impaired detoxification capacity. Investigating the neurobiological bases of impaired antioxidant capacity is thus a research priority in the pathophysiology of ASD. We measured the urinary levels of hexanoyl-lysine (HEL) which is a new oxidative stress biomarker, total antioxidant power (TAP) and DNA methylation biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG), and the plasma levels of superoxide dismutase (SOD), which is a major antioxidant enzyme. We examined whether the urinary levels of these enzymes and biomarkers may be related to symptoms of social impairment in 20 individuals with ASD (meanage,11.1±5.2years) and 12 age- and gender-matched healthy controls (meanage,14.3±6.2years). Symptoms of social impairment were assessed using the Social Responsiveness Scale (SRS). The dietary TAP of the fruit juice, chocolate, cookies, biscuits, jam and marmalade were significantly higher in the ASD group than in the control group, although the intake of nutrients was not significantly different between the groups. The urinary TAP levels were significantly lower in the ASD group than in the control group. There were no significantly differences in urinary HEL and 8-OHdG levels between the ASD and control groups. The SRS scores were significantly higher in the ASD group than in the control group. Stepwise regression analysis revealed that urinary TAP levels and plasma SOD levels can differences in the biomarkers and the SRS scores between the ASD group and the control group. The endogenous antioxidant capacity may be deficient without altered urinary HEL and 8-OHdG levels in individuals with ASD. The plasma SOD levels may be related to reduced endogenous antioxidant capacity.
患有自闭症谱系障碍（ASD）的个体解毒能力受损。因此，研究抗氧化能力受损的神经生物学基础是ASD病理生理学中的一个研究重点。我们测量了一种新的氧化应激生物标志物己酰赖氨酸（HEL）、总抗氧化能力（TAP）和DNA甲基化生物标志物8-羟基-2'-脱氧鸟苷（8-OHdG）的尿水平，以及主要抗氧化酶超氧化物歧化酶（SOD）的血浆水平。我们检查了这些酶和生物标志物的尿水平是否可能与20名ASD患者（平均年龄11.1±5.2岁）和12名年龄及性别匹配的健康对照者（平均年龄14.3±6.2岁）的社会功能损害症状有关。使用社会反应量表（SRS）评估社会功能损害症状。尽管两组之间的营养摄入量没有显著差异，但ASD组果汁、巧克力、饼干、曲奇、果酱和橘子酱的饮食TAP显著高于对照组。ASD组的尿TAP水平显著低于对照组。ASD组和对照组之间的尿HEL和8-OHdG水平没有显著差异。ASD组的SRS评分显著高于对照组。逐步回归分析显示，尿TAP水平和血浆SOD水平可以解释ASD组和对照组之间生物标志物和SRS评分的差异。ASD个体的内源性抗氧化能力可能不足，而尿HEL和8-OHdG水平没有改变。血浆SOD水平可能与内源性抗氧化能力降低有关。
