Meguid Nagwa A, Ghozlan Said A S, Mohamed Magda F, Ibrahim Marwa K, Dawood Reham M, Din Noha G Bader El, Abdelhafez Tawfeek H, Hemimi Maha, Awady Mostafa K El
Department of Research on Children with Special Needs, Medical Research Division, National Research Centre, Giza, Egypt.
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
Biomark Insights. 2017 Feb 23;12:1177271917691035. doi: 10.1177/1177271917691035. eCollection 2017.
The molecular basis of the pathophysiological role of oxidative stress in autism is understudied. Herein, we used polymerase chain reaction (PCR) array to analyze transcriptional pattern of 84 oxidative stress genes in peripheral blood mononuclear cell pools isolated from 32 autistic patients (16 mild/moderate and 16 severe) and 16 healthy subjects (each sample is a pool from 4 autistic patients or 4 controls). The PCR array data were further validated by quantitative real-time PCR in 80 autistic children (55 mild/moderate and 25 severe) and 60 healthy subjects. Our data revealed downregulation in , and transcripts (1.5, 3.8, 1.2, 1.7, and 2.2, respectively; < .05 for all) in autistic group compared with controls. In addition, and exhibited 1.4- and 1.7-fold downregulation, respectively, in severe autistic patients when compared with mild/moderate group ( = .005 and .0008, respectively). This study helps in a better understanding of the underlying biology and related genetic factors of autism, and most importantly, it presents suggested candidate biomarkers for diagnosis and prognosis purposes as well as targets for therapeutic intervention.
氧化应激在自闭症病理生理作用中的分子基础研究不足。在此，我们使用聚合酶链反应（PCR）芯片分析了从32名自闭症患者（16名轻度/中度和16名重度）和16名健康受试者中分离出的外周血单个核细胞池中的84个氧化应激基因的转录模式（每个样本是来自4名自闭症患者或4名对照的混合样本）。通过定量实时PCR在80名自闭症儿童（55名轻度/中度和25名重度）和60名健康受试者中进一步验证了PCR芯片数据。我们的数据显示，与对照组相比，自闭症组中 、 和 转录本下调（分别为1.5、3.8、1.2、1.7和2.2倍；均P <.05）。此外，与轻度/中度组相比，重度自闭症患者中 和 分别下调了1.4倍和1.7倍（分别为P = 0.005和P = 0.0008）。这项研究有助于更好地理解自闭症的潜在生物学和相关遗传因素，最重要的是，它提出了用于诊断和预后目的的候选生物标志物以及治疗干预的靶点。
