ESEI - Department of Computer Science, University of Vigo, Edificio Politécnico, Campus Universitario As Lagoas s/n, 32004 Ourense, Spain.
ESEI - Department of Computer Science, University of Vigo, Edificio Politécnico, Campus Universitario As Lagoas s/n, 32004 Ourense, Spain; CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Food Microbiol. 2016 Dec;60:137-41. doi: 10.1016/j.fm.2016.06.015. Epub 2016 Jun 29.
Bifidobacteria are gut commensal microorganisms belonging to the Actinobacteria group. Some specific strains of Bifidobacterium animalis subsp. lactis are used in functional foods as they are able to exert health-promoting effects in the human host. Due to the limited genetic variability within this subspecies, it is sometimes difficult for a manufacturer to properly track its strain once included in dairy products or functional foods. In this paper, we present a peptidome-based analysis in which the proteomes of a set of B. animalis subsp. lactis strains were digested in silico with human gut endopeptidases. The molecular masses were compared along all the strains to detect strain-specific peptides. These peptides may be interesting towards the development of methodologies for strain identification in the final product.
双歧杆菌是属于放线菌目的肠道共生微生物。一些特定的动物双歧杆菌亚种。乳双歧杆菌菌株被用于功能性食品，因为它们能够对人类宿主发挥有益健康的作用。由于该亚种内的遗传可变性有限，因此制造商有时很难在乳制品或功能性食品中包含其菌株后对其进行正确追踪。在本文中，我们进行了基于肽组学的分析，使用人类肠道内肽酶对一组动物双歧杆菌亚种。乳双歧杆菌菌株的蛋白质组进行了计算机模拟消化，并比较了所有菌株的分子量，以检测菌株特异性肽。这些肽在最终产品的菌株鉴定方法的开发中可能具有重要意义。
