乳双歧杆菌亚种的比较基因组学揭示了严格的双歧杆菌单系分类群。
Comparative genomics of Bifidobacterium animalis subsp. lactis reveals a strict monophyletic bifidobacterial taxon.
机构信息
Laboratory of Probiogenomics, Department of Life Sciences, University of Parma, Parma, Italy.
出版信息
Appl Environ Microbiol. 2013 Jul;79(14):4304-15. doi: 10.1128/AEM.00984-13. Epub 2013 May 3.
Abstract
Strains of Bifidobacterium animalis subsp. lactis are extensively exploited by the food industry as health-promoting bacteria, although the genetic variability of members belonging to this taxon has so far not received much scientific attention. In this article, we describe the complete genetic makeup of the B. animalis subsp. lactis Bl12 genome and discuss the genetic relatedness of this strain with other sequenced strains belonging to this taxon. Moreover, a detailed comparative genomic analysis of B. animalis subsp. lactis genomes was performed, which revealed a closely related and isogenic nature of all currently available B. animalis subsp. lactis strains, thus strongly suggesting a closed pan-genome structure of this bacterial group.
摘要
双歧杆菌亚种。乳杆菌菌株被食品工业广泛开发为具有促进健康作用的细菌，尽管属于该分类群的成员的遗传变异性迄今为止尚未受到太多科学关注。在本文中，我们描述了双歧杆菌亚种。乳杆菌 Bl12 基因组的完整遗传组成，并讨论了该菌株与属于该分类群的其他已测序菌株的遗传相关性。此外，还对双歧杆菌亚种。乳杆菌基因组进行了详细的比较基因组分析，结果表明所有现有的双歧杆菌亚种。乳杆菌菌株具有密切相关的同基因性质，这强烈表明该细菌组具有封闭的泛基因组结构。
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本文引用的文献
1
Host-microbe interactions that facilitate gut colonization by commensal bifidobacteria.促进共生双歧杆菌肠道定植的宿主-微生物相互作用。
Trends Microbiol. 2012 Oct;20(10):467-76. doi: 10.1016/j.tim.2012.07.002. Epub 2012 Aug 16.
2
Complete genome sequences of probiotic strains Bifidobacterium animalis subsp. lactis B420 and Bi-07.益生菌动物双歧杆菌乳酸亚种B420和Bi - 07的全基因组序列
J Bacteriol. 2012 Aug;194(15):4131-2. doi: 10.1128/JB.00766-12.
3
Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.Geneious Basic：一个集成和可扩展的桌面软件平台，用于组织和分析序列数据。
Bioinformatics. 2012 Jun 15;28(12):1647-9. doi: 10.1093/bioinformatics/bts199. Epub 2012 Apr 27.
4
PGAP: pan-genomes analysis pipeline.PGAP：泛基因组分析管道。
Bioinformatics. 2012 Feb 1;28(3):416-8. doi: 10.1093/bioinformatics/btr655. Epub 2011 Nov 29.
5
Short communication: the complete genome sequence of Bifidobacterium animalis subspecies animalis ATCC 25527(T) and comparative analysis of growth in milk with B. animalis subspecies lactis DSM 10140(T).短篇通讯：动物双歧杆菌亚种动物双歧杆菌 ATCC 25527(T) 的全基因组序列和与乳双歧杆菌亚种乳双歧杆菌 DSM 10140(T) 在牛奶中生长的比较分析。
J Dairy Sci. 2011 Dec;94(12):5864-70. doi: 10.3168/jds.2011-4499.
6
Probiogenomics as a tool to obtain genetic insights into adaptation of probiotic bacteria to the human gut.益生菌基因组学作为一种工具，用于深入了解益生菌对人类肠道的适应性的遗传机制。
Bioeng Bugs. 2012 Mar-Apr;3(2):73-9. doi: 10.4161/bbug.18540. Epub 2012 Mar 1.
7
Complete genome sequence of Bifidobacterium animalis subsp. lactis BLC1.乳双歧杆菌亚种 BLC1 的全基因组序列
J Bacteriol. 2011 Nov;193(22):6387-8. doi: 10.1128/JB.06079-11.
8
Genome sequence of the probiotic strain Bifidobacterium animalis subsp. lactis CNCM I-2494.双歧杆菌亚种乳双歧杆菌 CNCM I-2494 的基因组序列。
J Bacteriol. 2011 Oct;193(19):5560-1. doi: 10.1128/JB.05716-11.
9
Anti-inflammatory properties of probiotic bacteria on Salmonella-induced IL-8 synthesis in enterocyte-like Caco-2 cells.益生菌对沙门氏菌诱导的肠细胞样 Caco-2 细胞 IL-8 合成的抗炎作用。
Benef Microbes. 2010 Jun;1(2):121-30. doi: 10.3920/BM2009.0021.
10
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Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):11217-22. doi: 10.1073/pnas.1105380108. Epub 2011 Jun 20.
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