Cozzolino Immacolata, Rocco Monia, Villani Giancarlo, Picardi Marco
Department of Public Health, 'Federico II' University of Naples, Naples, Italy.
Acta Cytol. 2016;60(4):302-314. doi: 10.1159/000448389. Epub 2016 Aug 24.
In the last decades, lymph node fine-needle cytology (FNC), coupled with flow cytometry (FC), has gained a role in the diagnosis and classification of non-Hodgkin lymphoma (NHL). The combination of FNC/FC allows the diagnosis and classification of NHL in lymph node samples with a high sensitivity and specificity by combining cytological features and specific phenotypic profiles. The present review provides a brief technical description of FC and a detailed analysis of the current markers and their combinations (diagnostic algorithm) for the diagnosis and classification of NHL. The basic principles of clonality assessment, as well as the diagnostic strengths and weaknesses of the procedure, are reported. The current diagnostic algorithms for NHL classification are critically reviewed with a focus on specific problems related to single entities. Moreover, this review provides a detailed analysis of the different clinical contexts in which FNC/FC is performed and related implications. Future and further applications of FNC/FC for NHL are also discussed.
在过去几十年中，淋巴结细针穿刺细胞学检查（FNC）与流式细胞术（FC）相结合，在非霍奇金淋巴瘤（NHL）的诊断和分类中发挥了作用。FNC/FC的联合应用通过结合细胞学特征和特定的表型谱，能够以高灵敏度和特异性对淋巴结样本中的NHL进行诊断和分类。本综述简要介绍了FC的技术，并详细分析了目前用于NHL诊断和分类的标志物及其组合（诊断算法）。报告了克隆性评估的基本原则以及该检查方法的诊断优势和不足。对当前NHL分类的诊断算法进行了批判性回顾，重点关注与单个实体相关的特定问题。此外，本综述详细分析了进行FNC/FC的不同临床背景及其相关影响。还讨论了FNC/FC在NHL中的未来及进一步应用。
