Oszmiański Jan, Lachowicz Sabina
Department of Fruit and Vegetable Processing, Wroclaw University of Environmental and Life Science, 37, Chełmońskiego Street, Wroclaw 51-630, Poland.
Molecules. 2016 Aug 22;21(8):1098. doi: 10.3390/molecules21081098.
The aim of this study was to evaluate the production of dried fruits and juices from chokeberry as potential sources of bioactive compounds with beneficial effects on human health. Dry powders and juices from chokeberry were analyzed for the contents of sugars with high-performance liquid chromatography coupled with an evaporative light scattering detector (HPLC-ELSD method), and the antioxidant capacity was analyzed by the FRAP (ferric-reducing ability of plasma) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) tests. Polyphenols were identified by high performance liquid chromatography (HPLC) coupled with a tandem mass spectrometer and a photodiode-array detector (LC-PDA-ESI-MS/MS), and their quantitative analysis was carried out by UPLC-MS/MS (using a Q/TOF detector and a PDA detector). A total of 27 polyphenolic compounds was identified in chokeberry products, including 7 anthocyanins, 11 flavonols, 5 phenolic acids, 3 flavan-3-ols and 1 flavanone. Three anthocyanin derivatives were reported for the first time from chokeberry fruit. A higher activity of the bioactive compounds was determined in dried fruit pomace and in juice obtained from crushed fruits than in those from the whole fruits. In addition, the pomace was found to be a better material for the production of dry powders, compared to chokeberry fruits.
本研究的目的是评估将黑果腺肋花楸制成干果和果汁作为对人体健康有有益影响的生物活性化合物潜在来源的情况。采用高效液相色谱-蒸发光散射检测器联用（HPLC-ELSD法）分析黑果腺肋花楸的干粉和果汁中的糖含量，通过FRAP（血浆铁还原能力）和ABTS（2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸））试验分析抗氧化能力。通过高效液相色谱（HPLC）与串联质谱仪和光电二极管阵列检测器联用（LC-PDA-ESI-MS/MS）鉴定多酚类物质，并通过超高效液相色谱-质谱/质谱联用（使用Q/TOF检测器和PDA检测器）进行定量分析。在黑果腺肋花楸产品中总共鉴定出27种多酚类化合物，包括7种花青素、11种黄酮醇、5种酚酸、3种黄烷-3-醇和1种黄烷酮。首次从黑果腺肋花楸果实中报道了3种花青素衍生物。结果表明，与整果相比，干果果渣和破碎果实制得的果汁中生物活性化合物的活性更高。此外，与黑果腺肋花楸果实相比，果渣被发现是生产干粉的更好原料。
