Laboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria.
Institute of Biophysics of the Czech Academy of Sciences, Kralovopolska 135, 612 65 Brno, Czech Republic; Department of Animal Physiology and Immunology, Institute of Experimental Biology, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic.
Food Chem. 2019 Jun 30;284:108-117. doi: 10.1016/j.foodchem.2019.01.108. Epub 2019 Jan 23.
The current study reports data on antioxidant, antimicrobial and neutrophil-modulating activities of different polyphenolic preparations from black chokeberry fruits: crude extract, purified extract standardized to 20% and 40% anthocyanins, and proanthocyanidins; as well as pure compounds (chlorogenic acid, cyanidin-3-O-galactoside, epicatechin, rutin and quercetin) present in black chokeberries. Minor phenolic components - quercetin and epicatechin showed the highest ORAC and TRAP antioxidant activity. Given the amount of individual phenolics in the fruits, proanthocyanidins are the major contributor to antioxidant activity of fresh black chokeberries. Studied polyphenols and preparations had no effect on the spontaneous chemiluminescence (CL) of human neutrophils and only mild effect on PMA-activated CL. Greater effects were observed on OZP-activated CL, being statistically significant (p < 0.05) for quercetin and rutin. The antimicrobial activity test against 10 pathogens showed that black chokeberry proanthocyanidins are the most potent antimicrobial agents in the fruit.
本研究报告了黑果腺肋花楸果实中不同多酚制剂的抗氧化、抗菌和中性粒细胞调节活性的数据：粗提取物、标准化至 20%和 40%花青素的纯化提取物以及原花青素；以及黑果腺肋花楸中存在的纯化合物（绿原酸、矢车菊素-3-O-半乳糖苷、表儿茶素、芦丁和槲皮素）。 低酚类成分 - 槲皮素和表儿茶素具有最高的 ORAC 和 TRAP 抗氧化活性。 鉴于果实中个体酚类的含量，原花青素是新鲜黑果腺肋花楸抗氧化活性的主要贡献者。 研究的多酚和制剂对人中性粒细胞的自发化学发光 (CL) 没有影响，仅对 PMA 激活的 CL 有轻微影响。 在 OZP 激活的 CL 上观察到更大的影响，槲皮素和芦丁的影响具有统计学意义 (p < 0.05)。 对 10 种病原体的抗菌活性测试表明，黑果腺肋花楸原花青素是果实中最有效的抗菌剂。
