Department of Chemistry and Research Institute of Life Science, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Food Chem. 2014 Mar 1;146:1-5. doi: 10.1016/j.foodchem.2013.09.029. Epub 2013 Sep 12.
The type and content of plant polyphenols can be influenced by maturity. Korean chokeberry (Aronia melanocarpa) leaves of three different maturities (young, mature, and aged) were extracted with 70% aqueous methanol. The polyphenols in the leaves were analysed for the first time using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and comparison with reported data. Among the 12 characterised components, five flavonoids, 3, 4, and 10-12, and a dicaffeoylquinic acid derivative, 6, were characterised for the first time in chokeberry leaves. Each polyphenol component was validated and quantified using a representative polyphenol standard of the same group. The antioxidant activity of the three different mature leaf extracts was determined. The antioxidant activity was highest for young leaves, followed by mature and aged leaves. The results suggest that younger chokeberry leaves may be more favourable for processing a higher quality functional tea due to their higher polyphenol content.
植物多酚的类型和含量会受到成熟度的影响。本研究采用 70%甲醇水溶液提取三种不同成熟度（幼叶、成熟叶和陈叶）的韩国黑果腺肋花楸叶片中的多酚。采用高效液相色谱-串联质谱（HPLC-MS/MS）首次分析了叶片中的多酚，并与报道的数据进行了比较。在所鉴定的 12 种成分中，5 种类黄酮（3、4、10-12）和一种二咖啡酰奎宁酸衍生物（6）首次在黑果腺肋花楸叶片中被鉴定出来。每种多酚成分均使用同组的代表性多酚标准品进行了验证和定量。测定了三种不同成熟叶片提取物的抗氧化活性。结果表明，幼叶的抗氧化活性最高，其次是成熟叶和陈叶。这些结果表明，由于含有较高的多酚类物质，较年轻的黑果腺肋花楸叶片可能更有利于加工成更高品质的功能性茶。
