Jacomin Anne-Claire, Nezis Ioannis P
School of Life Sciences, University of Warwick, CV4 7AL, Coventry, UK.
Methods Mol Biol. 2016;1457:69-78. doi: 10.1007/978-1-4939-3795-0_5.
Oogenesis is a fundamental biological process for the transmission of genetic information to the next generations. Drosophila has proven to be a valuable model for elucidating the molecular and cellular mechanisms involved in this developmental process. It has been shown that autophagy participates in the maturation of the egg chamber. Here we provide a protocol for monitoring and quantification of the autophagic process in the Drosophila germline cells using the fluorescent reporters mCherry-DmAtg8a and GFP-mCherry-DmAtg8a.
卵子发生是将遗传信息传递给下一代的基本生物学过程。果蝇已被证明是阐明这一发育过程中涉及的分子和细胞机制的有价值模型。研究表明，自噬参与卵室的成熟。在这里，我们提供了一种使用荧光报告基因mCherry-DmAtg8a和GFP-mCherry-DmAtg8a监测和定量果蝇生殖系细胞自噬过程的方法。
