Blake Amelia J, Finger Danielle S, Hardy Victoria L, Ables Elizabeth T
East Carolina University, 1001 E. 10th Street, Mailstop 551, Greenville, NC, 27858, USA.
Methods Mol Biol. 2017;1622:161-184. doi: 10.1007/978-1-4939-7108-4_13.
Elucidating the full repertoire of molecular mechanisms that promote stem cell maintenance requires sophisticated techniques for identifying and characterizing gene function in stem cells in their native environment. Ovarian germline stem cells in the fruit fly, Drosophila melanogaster, are an ideal model to study the complex molecular mechanisms driving stem cell function in vivo. A variety of new genetic tools make RNAi a useful complement to traditional genetic mutants for the investigation of the molecular mechanisms guiding ovarian germline stem cell function. Here, we provide a detailed guide for using targeted RNAi knockdown for the discovery of gene function in ovarian germline stem cells and their progeny.
要阐明促进干细胞维持的完整分子机制，需要精密的技术来识别和表征天然环境中干细胞的基因功能。果蝇卵巢中的生殖系干细胞是研究体内驱动干细胞功能的复杂分子机制的理想模型。多种新的遗传工具使RNA干扰成为传统遗传突变体的有用补充，用于研究指导卵巢生殖系干细胞功能的分子机制。在这里，我们提供了一份详细指南，介绍如何利用靶向RNA干扰敲低技术来发现卵巢生殖系干细胞及其后代中的基因功能。
