用于检测植物核小体上表观遗传标记的染色质免疫沉淀法

Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes.

作者信息

Nagaki Kiyotaka

机构信息

Institute of Plant Science and Resources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan.

出版信息

Methods Mol Biol. 2016;1469:197-206. doi: 10.1007/978-1-4939-4931-1_16.

DOI:10.1007/978-1-4939-4931-1_16

PMID:27557697

Abstract

Due to high resolution and reproducibility, chromatin immunoprecipitation (ChIP) has been used as a standard tool to investigate epigenetic marks including modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.

摘要

由于具有高分辨率和可重复性，染色质免疫沉淀（ChIP）在过去十年中一直被用作研究表观遗传标记的标准工具，这些标记包括修饰的组蛋白和特定的组蛋白变体（例如，着丝粒特异性组蛋白H3，CENH3）。在此，我描述了一种适用于多种植物物种的灵敏且背景低的ChIP方案。