State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
Sci Rep. 2016 Aug 25;6:32066. doi: 10.1038/srep32066.
Biofilm is recognized as a contributing factor to the capacity of Acinetobacter baumannii to persist and prosper in medical settings, but it is still unknown whether biofilms contribute to the spread of A. baumannii. In this study, the biofilm formation of 114 clinical A. baumannii isolates and 32 non-baumannii Acinetobacter isolates was investigated using a microtiter plate assay. The clonal relationships among A. baumannii isolates were assessed using pulsed-field gel electrophoresis and multilocus sequence typing, and one major outbreak clone and 5 other epidemic clones were identified. Compared with the epidemic or outbreak A. baumannii isolates, the sporadic isolates had significantly higher biofilm formation, but no significant difference was observed between the sporadic A. baumannii isolates and the non-baumannii Acinetobacter isolates, suggesting that biofilm is not important for the epidemic spread of A. baumannii. Of the multidrug-resistant (MDR) A. baumannii isolates in this study, 95.7% were assigned to international clone 2 (IC2) and showed significantly lower biofilm formations than the other isolates, suggesting that biofilm did not contribute to the high success of IC2. These findings have increased our understanding of the potential relationship between biofilm formation and the epidemic capacity of A. baumannii.
生物膜被认为是鲍曼不动杆菌在医疗环境中持续存在和繁殖的一个因素，但目前尚不清楚生物膜是否有助于鲍曼不动杆菌的传播。在这项研究中，使用微量滴定板测定法研究了 114 株临床鲍曼不动杆菌分离株和 32 株非鲍曼不动杆菌不动杆菌分离株的生物膜形成情况。使用脉冲场凝胶电泳和多位点序列分型评估了鲍曼不动杆菌分离株之间的克隆关系，并鉴定出一个主要暴发克隆和 5 个其他流行克隆。与流行或暴发的鲍曼不动杆菌分离株相比，散发的分离株具有明显更高的生物膜形成，但散发的鲍曼不动杆菌分离株与非鲍曼不动杆菌分离株之间没有观察到显著差异，这表明生物膜对鲍曼不动杆菌的流行传播并不重要。在本研究中，耐多药（MDR）鲍曼不动杆菌分离株的 95.7% 被分配到国际克隆 2（IC2），其生物膜形成明显低于其他分离株，这表明生物膜并没有促成 IC2 的高成功率。这些发现增加了我们对生物膜形成与鲍曼不动杆菌流行能力之间潜在关系的理解。
