Clinton Michael, Nandi Sunil, Zhao Debiao, Olson Sarah, Peterson Patrick, Burdon Tom, McBride Derek
Developmental Biology, The Roslin Institute and R(D)SVS, University of Edinburgh, Roslin, UK.
Sex Dev. 2016;10(4):210-216. doi: 10.1159/000448502. Epub 2016 Aug 25.
The chicken embryo is an established model system for studying early vertebrate development. One of the major advantages of this model is the facility to perform manipulations in ovo and then continue incubation and observe the effects on embryonic development. However, in common with other vertebrate models, there is a tendency to disregard the sex of the experimental chicken embryos, and this can lead to erroneous conclusions, a lack of reproducibility, and wasted efforts. That this neglect is untenable is emphasised by the recent demonstration that avian cells and tissues have an inherent sex identity and that male and female tissues respond differently to the same stimulus. These sexually dimorphic characteristics dictate that analyses and manipulations involving chicken embryos should always be performed using tissues/embryos of known sex. Current sexing protocols are unsuitable in many instances because of the time constraints imposed by most in ovo procedures. To address this lack, we have developed a real-time chicken sexing assay that is compatible with in ovo manipulations, reduces the number of embryos required, and conserves resources.
鸡胚是研究早期脊椎动物发育的成熟模型系统。该模型的主要优势之一是便于在卵内进行操作，然后继续孵化并观察对胚胎发育的影响。然而，与其他脊椎动物模型一样，实验鸡胚的性别往往被忽视，这可能导致错误的结论、缺乏可重复性以及精力的浪费。最近的研究表明，禽类细胞和组织具有内在的性别特征，并且雄性和雌性组织对相同刺激的反应不同，这强调了这种忽视是站不住脚的。这些性别二态性特征表明，涉及鸡胚的分析和操作应始终使用已知性别的组织/胚胎进行。由于大多数卵内操作有时间限制，目前的性别鉴定方案在许多情况下并不适用。为了解决这一不足，我们开发了一种实时鸡性别鉴定方法，该方法与卵内操作兼容，减少了所需胚胎的数量，并节省了资源。
