Anthony D J, Profio A E, Balchum O J
Photochem Photobiol. 1989 May;49(5):583-6. doi: 10.1111/j.1751-1097.1989.tb08427.x.
The fluorescence emission spectra from human bronchial mucosa and tumors, before and after injection of dihematoporphyrin ether/ester, have been measured with an optical multichannel analyzer from 500 to 750 nm. Fluorescence was excited with a violet krypton ion laser (average wavelength 410 nm). The autofluorescence spectra decrease monotonically with increasing wavelength except for a small broad peak near 600 nm. The spectra from tumor sites, after injection of the fluorescent porphyrin, exhibit the characteristic fluorescence emission at 630 and 690 nm, added to the autofluorescence spectrum. The spectra from control or nontumor sites are similar but the magnitude of the component due to the injected porphyrin is smaller than at a tumor site. The magnitude ratio of tumor to control site fluorescence depends on concentration of the porphyrin, tumor thickness, and time after injection. Autofluorescence degrades contrast and thus makes very thin tumors difficult to image. Subtraction of the autofluorescence background is desirable.
使用光学多通道分析仪在500至750纳米范围内测量了注射二血卟啉醚/酯前后人支气管黏膜和肿瘤的荧光发射光谱。用紫色氪离子激光（平均波长410纳米）激发荧光。除了在600纳米附近有一个小的宽峰外，自体荧光光谱随波长增加单调下降。注射荧光卟啉后，肿瘤部位的光谱在630和690纳米处呈现出特征性荧光发射，并叠加在自体荧光光谱上。对照或非肿瘤部位的光谱相似，但由于注射卟啉产生的成分的强度比肿瘤部位小。肿瘤与对照部位荧光的强度比取决于卟啉的浓度、肿瘤厚度和注射后的时间。自体荧光会降低对比度，因此使非常薄的肿瘤难以成像。减去自体荧光背景是可取的。
