König K, Flemming G, Hibst R
Institute of Anatomy/Anatomy II, Friedrich Schiller University Jena, Germany.
Cell Mol Biol (Noisy-le-grand). 1998 Dec;44(8):1293-300.
We studied spectral autofluorescence characteristics of dental caries. A wide range of carious lesions revealed characteristic emission of endogenous fluorophores with strong fluorescence bands in the red spectral region when excited with 407 nm line radiation of a krypton ion laser. Healthy hard dental tissue exhibited no emission bands in the red. The fluorescence spectra, fluorescence excitation spectra as well as the reflectance spectra of carious lesions were found to be typical for fluorescent porphyrins, mainly protoporphyrin IX. A possible source of these porphyrins within carious tissues is bacterial biosynthesis. Non-invasive sensitive in vivo caries detection by means of appropriate excitation sources and porphyrin fluorescence detectors should be possible.
我们研究了龋齿的光谱自体荧光特性。当用氪离子激光的407nm谱线辐射激发时，多种龋损显示出内源性荧光团的特征发射，在红色光谱区域有强荧光带。健康的硬牙组织在红色区域没有发射带。发现龋损的荧光光谱、荧光激发光谱以及反射光谱是荧光卟啉的典型特征，主要是原卟啉IX。龋损组织中这些卟啉的一个可能来源是细菌生物合成。通过适当的激发源和卟啉荧光探测器进行非侵入性灵敏的体内龋齿检测应该是可行的。
