He Li-Hua, Li Jiao, Deng Yuan-Xiong, Zhang Xiao-Jie, Chen Rong, Lv Yuan
Department of Pharmaceutical Science, Medical College of Hunan Normal University, Changsha 410013, PR China.
Department of Pharmaceutical Science, Medical College of Hunan Normal University, Changsha 410013, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Oct 15;1033-1034:180-186. doi: 10.1016/j.jchromb.2016.08.030. Epub 2016 Aug 20.
Fructus Gradeniae, the fruit of Gardenia jasminoides Ellis, was used alone or in combination with other herb medicines in the treatment of type 2 diabetes mellitus in China for a long time. In present investigation, the HPLC method for the determination of geniposide in rat plasma was developed and validated, and the pharmacokinetics of geniposide in type 2 diabetic rats after oral administration of Fructus Gradeniae extract or pure was studied. The results showed that the pharmacokinetic profile (especially the area under the plasma concentration-time curve, AUC) of geniposide in type 2 diabetic rats after orally administered with Fructus Gradeniae extract or pure geniposide was remarkably different from that in normal rats. The results indicated that the increased AUC of geniposide in type 2 diabetic rats did not result from the effects of other components contained in Fructus Gradeniae. It could be speculated that the increased AUC of geniposide might result from the pathological state of type 2 diabetes mellitus which resulted in the pharmacokinetic alterations of geniposide.
栀子,即栀子(Gardenia jasminoides Ellis)的果实,在中国长期单独或与其他草药联合用于治疗2型糖尿病。在本研究中,建立并验证了大鼠血浆中栀子苷的HPLC测定方法,并研究了2型糖尿病大鼠口服栀子提取物或纯栀子苷后栀子苷的药代动力学。结果表明,2型糖尿病大鼠口服栀子提取物或纯栀子苷后栀子苷的药代动力学特征(尤其是血浆浓度-时间曲线下面积,AUC)与正常大鼠显著不同。结果表明,2型糖尿病大鼠栀子苷AUC的增加并非由栀子中所含其他成分的作用所致。可以推测,栀子苷AUC的增加可能是由于2型糖尿病的病理状态导致栀子苷药代动力学改变所致。