Department of Herbology, College of Korean Medicine, Daegu Haany University, 136, Shinchendong-ro, Suseong-gu, Deagu 42158, Republic of Korea.
Department of Beauty Design, Hosan University, Gyeongsan, Republic of Korea.
Biomed Res Int. 2020 Feb 24;2020:4826176. doi: 10.1155/2020/4826176. eCollection 2020.
Gardeniae Fructus 50% EtOH extract (GE) is a traditional herb that has been used to treat a variety of diseases. In this study, we investigate the antioxidant, anti-inflammatory, and antiapoptotic properties of GE on acute reflux-induced esophagitis (RE) model in rats. 2,2'-Azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays were performed to determine the antioxidant activity of GE. GE was given orally at 50 and 100 mg/kg body weight 1h 30 min prior to RE induction. And its effect was assessed in comparison with RE control and normal groups. The administration of the extract of the GE showed remarkable protection of mucosal damage in esophageal tissue, and the histologic observation showed that the gastric lesion was improved. Increased reactive oxygen species (ROS) levels in the serum were diminished by GE treatment. The antioxidative biomarkers including nuclear factor-erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPX) were significantly increased. GE administration significantly reduced the inflammatory protein expression through MAPK-related signaling pathways and the nuclear factor-kappa B (NF-B) pathway. These results suggest that GE protects the esophagus mucosal membrane by attenuating oxidative stress and inflammatory response under reflux esophagitis condition through the antioxidant pathway. Therefore, it is suggested that GE may be a potential remedy for the treatment of reflux esophagitis.
栀子 50%乙醇提取物（GE）是一种传统草药，已被用于治疗多种疾病。在这项研究中，我们研究了 GE 对大鼠急性反流性食管炎（RE）模型的抗氧化、抗炎和抗凋亡作用。通过 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）和 2,2-二苯基-1-苦基肼（DPPH）自由基清除实验来测定 GE 的抗氧化活性。在诱导 RE 前 1 小时 30 分钟，以 50 和 100mg/kg 体重的剂量经口给予 GE，并与 RE 对照组和正常组进行比较，评估其效果。GE 的提取物给药显示出对食管组织黏膜损伤的显著保护作用，组织学观察显示胃损伤得到改善。血清中活性氧（ROS）水平的增加被 GE 处理所减弱。抗氧化生物标志物，包括核因子-红细胞 2 相关因子 2（Nrf-2）、血红素加氧酶-1（HO-1）、超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GPX），均显著增加。GE 通过 MAPK 相关信号通路和核因子-κB（NF-κB）通路减少了炎症蛋白的表达。这些结果表明，GE 通过抗氧化途径减轻氧化应激和炎症反应，从而保护食管黏膜免受反流性食管炎的影响。因此，GE 可能是治疗反流性食管炎的一种潜在药物。
