Yang Xuejing, Li Jin, Yang Xi, He Jun, Chang Yan-Xu
School of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang 150076, China.
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193 Tianjin, China.
Evid Based Complement Alternat Med. 2020 May 6;2020:1642761. doi: 10.1155/2020/1642761. eCollection 2020.
The extract of (GF) with different processing methods processed the different medicinal properties and efficacy. Crude GF (CGF) could be processed into stir-frying GF (SGF), gancao mix-frying GF (GCGF), and ginger mix-frying GF (GIGF) in practice. An LC-MS/MS method was established for simultaneous quantification of geniposidic acid, geniposide, genipin-1--gentiobioside, genipin, and crocetin in the rat plasma. The LLOQs for determination of all five components were 10 ng/mL. The accuracies of intraday and interday were in the range of 91%-105%. The recoveries of 5 analytes ranged from 81.0% to 114% with RSD less than 14%. The results showed that the AUCs (area under the plasma concentration-time curve) and (maximum plasma concentration) of geniposidic acid, genipin-1--gentiobioside, and geniposide after oral administration of the CGF extract were apparently higher than those after oral administration of other processed extracts. of geniposide in plasma after administration of GIGF significantly decreased ( < 0.01). Genipin was not detected in rat plasma after administration of the GIGF extract, but it can be detected in plasma after administration of CGF, SGF, and GCGF extract. Furthermore, crocin I and crocin II were not detected in plasma samples. Crocetin had higher concentration in rat plasma versus lower contents in extract. It was demonstrated that the different processing methods might influence the pharmacokinetics of geniposidic acid, genipin-1--gentiobioside, geniposide, genipin, and crocetin.
不同炮制方法的栀子提取物具有不同的药用特性和功效。生栀子（CGF）在实际应用中可炮制成炒栀子（SGF）、甘草制栀子（GCGF）和姜制栀子（GIGF）。建立了一种LC-MS/MS方法，用于同时定量大鼠血浆中的京尼平苷酸、栀子苷、京尼平-1-龙胆双糖苷、京尼平和西红花酸。测定所有五种成分的最低定量限均为10 ng/mL。日内和日间精密度在91%-105%范围内。5种分析物的回收率在81.0%至114%之间，相对标准偏差小于14%。结果表明，口服CGF提取物后，京尼平苷酸、京尼平-1-龙胆双糖苷和栀子苷的血浆浓度-时间曲线下面积（AUC）和最大血浆浓度（Cmax）明显高于口服其他炮制提取物后的相应值。给予GIGF后，血浆中栀子苷的Cmax显著降低（P<0.01）。给予GIGF提取物后，大鼠血浆中未检测到京尼平，但给予CGF、SGF和GCGF提取物后可在血浆中检测到。此外，血浆样品中未检测到西红花苷I和西红花苷II。西红花酸在大鼠血浆中的浓度较高，而在提取物中的含量较低。结果表明，不同的炮制方法可能会影响京尼平苷酸、京尼平-1-龙胆双糖苷、栀子苷、京尼平和西红花酸的药代动力学。
